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玉米单倍体诱导DH系的分子鉴定研究
Molecular Identification of Maize Induced Haploid DH Lines
【摘要】 采用36个SSR引物对先玉335的418个DH系进行分子鉴定,并与田间形态性状调查结果对比。结果表明:DNA指纹技术是鉴定DH系材料是否完全纯合的有效手段,仅通过田间表型鉴定很难准确判断材料是否纯合。规范的加倍扩繁程序是解决DH系较高混杂状况的根本措施。
【Abstract】 Four hundred and eighteen doubled haploids of Xianyu335 were identified with 36 SSR primers.The molecular data were compared with morphological data in field.It indicated that DNA technology was a useful method to identify the purity of DH lines,and standardized program from doubling to propagating was the ultimate way to reduce high impure rate of DH lines.
【基金】 “973”计划项目(2009CB118405);国家科技支撑计划项目(2007BAD31B01)
- 【文献出处】 玉米科学 ,Journal of Maize Sciences , 编辑部邮箱 ,2011年01期
- 【分类号】S513.037
- 【被引频次】11
- 【下载频次】529