【摘要】 目的:研究细胞分化剂CDA-Ⅱ(cell differentiation agent-Ⅱ)抑制人肺腺癌细胞A549增殖及对癌基因C-erbB2表达的影响。方法:不同浓度的CDA-Ⅱ作用人肺癌细胞A549,用CCK-8法检测细胞增殖抑制率;RT-PCR和Western blot检测经CDA-Ⅱ作用后A549细胞C-erbB2表达的改变。结果:CDA-Ⅱ能明显抑制肺腺癌细胞增殖,且抑制效应呈剂量依赖性。C-erbB2在CDA-Ⅱ抗人肺癌细胞A549增殖中表达下调。结论:CDA-Ⅱ可有效抑制人肺癌细胞A549增殖,该作用可能与抑制C-erbB2的信号传导通路有关。更多还原

【Abstract】 AIM: To investigate the effects of CDA-Ⅱ(Uroacitide) which is a healthy human urine extraction on proliferation and C-erbB2 expression of human lung cancer cell line A549. METHODS: The effect of CDA-Ⅱ on cellular viability was determined by CCK-8 assay.The change of C-erbB2 expression in A549 cells induced by CDA-Ⅱ was detected by RT-PCR and Western blot.RESULTS:After A549 cells were treated with various concentration of CDA-Ⅱ,cell proliferation was significantly inhibited in a dose-dependent manner.The expression of C-erbB2 was downregulated in A549 cells induced by CDA-Ⅱ.CONCLUSION: CDA-Ⅱ can inhibit the growth of A549 cells associated with downregulation of C-erbB2,which indicates that C-erbB2 is involved in CDA-Ⅱ induced proliferation inhibition of A549 cells.更多还原