【摘要】 目的研究姜黄素对人卵巢癌细胞迁移的影响及可能机制。方法培养人卵巢癌CaOV3细胞,用姜黄素、表皮生长因子(EGF)、小干扰RNA(siRNA)水通道蛋白3(AQP3)等不同途径处理细胞后,蛋白质免疫印迹实验检测AQP3表达,细胞迁移实验分析细胞的迁移能力。结果姜黄素呈浓度和时间依赖性地下调AQP3表达(P<0.05);并且姜黄素s、iRNA AQP3抑制人卵巢癌细胞迁移(P<0.05)。结论姜黄素通过降低AQP3表达,从而抑制肿瘤细胞迁移。更多还原

【Abstract】 Objective To investigate the effect and possible mechanism of curcumin on cell migration in human ovarian cancer.Methods Human ovarian cancer cells(CaOV3 cells) were cultured and treated with curcumin,epidermal growth factor(EGF) or small interfering RNA(siRNA) aquaporins 3(AQP3).AQP3 expression was detected by Western blot.Cell migration was analyzed by phagokinetic track motility assay.Results AQP3 expression was decreased by curcumin in both concentration-and time-dependent manners(P<0.05).Moreover,cell migration was inhibited by curcumin and siRNA AQP3(P<0.05).Conclusion Curcumin can inhibit cancer cell migration via down-regulating AQP3 expression.更多还原