【摘要】 目的观察烟草烟雾对大鼠离体胸主动脉环张力的影响,并探讨其作用机制。方法建立大鼠吸烟模型,采用离体胸主动脉环灌流装置,观察短期大量吸烟(SS)、中期大量吸烟(MS)及长期大量吸烟(LS)对氯化钾(KCl)、苯肾上腺素(PE)诱导血管收缩的作用,以及对乙酰胆碱(Ach)舒张血管环的影响,同时观察N-硝基-L-精氨酸甲酯(L-NAME)预处理对PE收缩血管作用的影响,采用Griess法测定各干预组血管环一氧化氮(NO)含量。结果与对照(CON)组相比,MS组、LS组胸主动脉对KCl及PE的收缩反应明显增强(P<0.05),对Ach舒张反应明显降低(P<0.05),血管NO含量显著降低,并且其作用强度与吸烟时间呈正比;血管环对KCl和PE最高浓度的收缩反应与NO的含量呈负相关。结论烟雾熏吸可使大鼠离体胸主动脉环收缩功能增强,内皮依赖性舒张功能减弱,血管功能的改变可能与NO含量的变化有关。更多还原

【Abstract】 Objective To investigate the effect of tension of thoracic aortas in smoking rats,and to explore its mechanism.Methods Rats models of smoking were established,and isolated rat thoracic aortic perfusion apparatus were used.Effects of short-term heavy smoking(SS),medium term heavy smoking(MS)and long-term heavy smoking(LS)on vasoconstriction induced by KCl,phenephrine(PE) were observed,as well as aceylcholine(Ach) on relaxation of vessel rings.The roles of preconditioning with NG-Nitro-L-Arginine Methyl Ester(L-NAME) on contraction by PE were also evaluated.Nitric oxide(NO) content in vessel rings of intervention group was measured with Griess method.Results Compared with control(CON) group,the contractile response of thoracic aortas which induced by KCl or PE increased significantly in LS group and MS group(P<0.05),in addition,the relative relaxation response induced by Ach and serum NO content decreased significantly in LS group and MS group(P<0.05),vasoconstriction which induced by KCl and maximal concentration of PE had significant negative correlation with NO content in blood vessels,furthermore,its intensity was proportional to the duration of smoking.Conclusion The vasoconstriction response of arteries in smoking rats increases,while the endothelial function and NO content in blood vessels decrease.NO may play an important role in the change of arterial function in cigarette smoking rats.更多还原