Genetic transformation is a valuable tool for direct crop improvement and functional genomics study.Unfortu-nately,wheat is considered as a recalcitrant plant to genetic transformation due to its low efficiency and genotype depend-ency.To overcome these problems,various transformation methods such as biolistic bombardment,Agrobacterium tumefa-ciens,pollen-tube pathway,ion implantation,laser microbeams puncture,treatment with polyethylene glycol and ultrasonic wave,and electroporation have been reported in w...