【摘要】 目的研究神经调节素1β(neuregulin1β,NRG1β)对实验性老年痴呆大鼠脑组织白细胞介素-21β(IL-21β)表达的影响。方法成年健康Wistar大鼠30只,经左侧脑室微量注射淀粉样蛋白β1-40(Aβ1-40)建立实验性痴呆模型,经右侧脑室注射NRG1β干预治疗,Y型电迷宫检测大鼠的认知功能,原位TUNEL法检测神经细胞凋亡,免疫组织化学法检测IL-21β表达。结果造模成功后,模型组大鼠较对照组认知能力明显下降、TUNEL阳性细胞数明显增多、神经细胞IL-21β蛋白表达增强(P<0.05)。经NRG1β治疗后,治疗组大鼠较模型组认知能力显著改善、TUNEL阳性细胞显著减少、神经细胞IL-21β表达减弱(P<0.05)。结论 NRG1β可能下调IL-21β表达,发挥抗炎作用,从而改善动物的学习记忆能力。更多还原

【Abstract】 Objective To investigate the effect of neuregulin 1β(NRG1β) on the neuronal apoptosis and the expressions of IL-21β in experimental dementia model rats.Methods Thirty adult healthy Wister rats were randomly divided into control group,model group and treated group consisting of 10 rats.The dementia models were established by injecting beta-amyloid protein 1-40(A β1-40) into the left lateral ventricle,and treated by injecting NRG1β into right lateral ventricle.The cognitive capacity of rats was evaluated with Y-electric maze.The neuronal apoptosis was counted by TUNEL assay.The expressions of IL-21β were determined with immunohistochemistry assay.Results The cognitive ability in model group rats reduced along with the number of neuronal apoptosis and the expressions of IL-21β increased significantly than those in control group(P <0.05).After treatment with NRG1β,the cognitive ability of rats improved,the number of neuronal apoptosis reduced and the expression of IL-21β decreased significantly than those in model group(P <0.05).Conclusion NRG1β could play an anti-inflammation by regulating the expressions of IL-21β to improve the capacity of learning and memory in experimental dementia rats.更多还原