【摘要】 目的研究小分子肽(KP)对小鼠成骨前体细胞MC3T3-E1增殖分化及NF-κB p50、p65表达的影响。方法 MTT法检测不同深度KP(0.01、0.1、1.0、10.0、25.0、50.0、100 mg.L-1)对MC3T3-E1生长的影响:PNPP法检测KP作用9、12、15 d后碱性磷酸酶活性:紫外分光光度法检测KP作用12、24 d后细胞中羟脯氨酸的含量;茜素红染色检测矿化结节;Western blot检测成骨矿化过程中细胞不同阶段NF-κB亚基p65和p50的表达。结果浓度为25.0、50.0、100mg.L-1的KP能促进MC3T3-E1细胞增殖生长;50、100 mg.L-1的KP作用细胞9、12、15 d和12、24 d后能分别使细胞中的碱性磷酸酶活性及羟脯氨酸含量高于对照组;30 d后KP组出现典型的矿化骨结节;作用3、12、24、30 d后细胞中p65及p50表达均出现不同程度下降。结论小分子肽(KP)可能是通过抑制NF-κB活性来促进MC3T3-E1细胞增殖分化。更多还原

【Abstract】 Aim To investigate the effect of the small molecular weight peptide(KP)on the proliferation and differentiation of mouse MC3T3-E1 cells and the expression of NF-κB p50,p65.Methods The cells growth,alkaline phosphatase(ALP)activity,hydroxyproline content and mineralization of MC3T3-E1 were measured by MTT,PNPP,visible spectrophotometry and alizarin red staining respectively.p65,p50 protein expression in MC3T3-E1 cells were detected by Western blot.Results KP enhanced cell proliferation after MC3T3-E1 cells was treated with KP(25.0,50.0,100 mg·L-1)for 24,48 h and 72 h.Treated with KP at concentrations of 50.0 and 100 mg·L-1 for 9,12,15 d,ALP activity was significantly stimulated;and hydroxyproline contents was enhanced after being treamted 12,24 d.KP had significant effect on mineralization at concentrations of 50.0 mg·L-1 and 100 mg·L-1.After 50.0 mg·L-1 and 100 mg·L-1 KP treatment for 3,12,24,30 d,the expressions of p65 and p50 in MC3T3-E1 cells were gradually decreased.Conclusion KP induct MC3T3-E1 cells proliferation and differentiation by inhibition of the NF-κB pathway.更多还原