【摘要】 目的观察淫羊藿苷对去卵巢大鼠骨和下丘脑不同核团ERβmRNA表达的影响,探讨其治疗绝经后骨质疏松的作用机制。方法将10～11月龄SD♀大鼠60只,随机分为假手术组、去卵巢模型组、淫羊藿苷小、中、大剂量组(每组12只)。以双侧卵巢切除法建立大鼠骨质疏松模型。分别用淫羊藿苷小、中、大剂量给药4个月,采用RT-PCR法,观察各组大鼠骨和下丘脑室旁核、视上核、弓状核ERβmRNA表达的变化。结果大鼠卵巢切除后,血清E2水平、椎骨骨密度、子宫湿重、胫骨和下丘脑弓状核ERβmRNA表达明显降低(P<0.01),下丘脑室旁核、视上核ERβmRNA表达均明显升高(P<0.01)。与去卵巢模型组比较,淫羊藿苷50.0和100.0 mg.kg-1组治疗后,血清E2水平、椎骨骨密度、胫骨和下丘脑弓状核ERβmRNA表达明显升高(P<0.01),下丘脑室旁核、视上核ERβmRNA表达均明显降低(P<0.01),子宫湿重无明显改变(P>0.05)。结论淫羊藿苷可以改善切除卵巢所致的骨质疏松大鼠的骨密度,对子宫无不良反应。其机制可能与其选择性调节去卵巢骨质疏松大鼠下丘脑不同核团ERβmRNA表达水平有关,调节下丘脑功能活动是其途径之一。更多还原

【Abstract】 Aim To observe the effects of icariin on the expression of the estrogen receptor beta(ERβ) mRNA of bone and different hypothalamic nuclei in ovariectomy Sprague-Dawley(SD) rats,and to investigate the mechanism of the treatment of postmenopausal osteoporosis with icariin.Methods Sixty female SD rats,aged ten to eleven months old,were divided randomly into sham operation group,ovariectomy model group and icariin 25.0,50.0 and 100.0 mg·kg-1 group(n=12 foreach).Ovariectomy bilaterally was used to establish the postmenopausal osteoporosis animal model.These rats were fed with icariin 25.0,50.0 and 100.0 mg·kg-1 for 4 months respectively.The semi-quantitative RT-PCR was performed to determine expressions of ERβ mRNA in bone and paraventricular hypothalamic nucleus(PVN),supraoptic hypothalamic nucleus(SUP) and arcuate hypothalamic nucleus(ARC).Results In the model group,serum estridiol level,bone mineral density(BMD)of vertebra,wet weight of uterus and the expressions of ERβ mRNA level of tibia and arcuate hypothalamic nucleus dropped significantly(P<0.01),but the expressions of ERβ mRNA in PVN and those of SUP were increased significantly(P<0.01).Compared with the model group,in rats after treatment with icariin(50.0 and 100.0 mg·kg-1),serum estridiol level,BMD of vertebra and the expressions of ERβ mRNA level of tibia and ARC were increased significantly(P<0.01),but the expressions ERβ mRNA in PVN and those of SUP were reduced significantly(P<0.01),and wet weight of uterus had no significant change(P>0.05).Conclusions Icariin can improve BMD of vertebra of the ovariectomy rats,without side effects on the uterus.The mechanism lies possibly in modulating the expressions of ERβ mRNA in different hypothalamic nuclei selectively in ovariectomy rats,which possibly is one of the ways for modulating the hypothalamic activity.更多还原