【摘要】 目的 研究缺氧诱导因子-1α(HIF-1α)反义寡核苷酸(asODN)转染的树突状细胞(DC)与U251胶质瘤细胞融合后的抗胶质瘤活性。方法 将HIF-1α asODN利用脂质体包裹转染DC,采用PEG化学融合方法将DC与U251胶质瘤细胞融合,通过MTT法及流式细胞仪检测胶质瘤细胞凋亡率。结果 HIF-1α asODN转染DC组与各对照组相比细胞增殖下降,凋亡增加(P<0.01)。结论 HIF-1α asODN转染DC后,再与U251胶质瘤细胞融合能够比单纯使用HIF-1α asODN转染U251细胞或单纯使用DC细胞取得更好的抑制胶质瘤细胞生长及促进其凋亡的效果。更多还原

【Abstract】 Objective To antisense oligodeoxynucleotides(asODN) targeting hypoxia inducible factor-1α gene into dendritic cells(DC),then fuse with glioma cell line U251 and to observe its effect of apoptosis inducing and growth restraining to U251 cells.Method The asODN targeting hypoxia inducible factor-1α gene was transduced into DC by liposomes mediated transfection.Then the transduced DCs are fused with glioma cells.The growth of U251 cell was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) method.The change of cells apoptosis rate was measured by flow cytometry.Results MTT method revealed that the cell growth in the transfected group was inhibited most significantly(P <0.01).Flow cytometry analysis revealed glioma cell line U251 suffered a more notable apoptosis than other group(P <0.01).Conclusion After transduction of HIF-1α asODN into DC,the transduced DC can inhibited human glioma cell line U251 in vitro remarkably and the apoptosis of U251 cell can be induced significantly.更多还原