【摘要】 目的 通过观察氟马西尼对异氟烷遗忘作用的影响,揭示异氟烷遗忘机制与苯二氮?受体的关系。方法 将48只小鼠随机分成6组,采用避暗实验观察腹腔注射不同剂量氟马西尼(0.5、1.0 mg/kg)对异氟烷处理小鼠步入潜伏期和错误次数的影响。结果 与生理盐水组相比,氟马西尼单用组小鼠的步入潜伏期和错误次数均无明显差异(P>0.05);异氟烷单用或合用组步入潜伏期明显缩短(P<0.05),错误次数明显增加(P<0.05或P<0.01)。与异氟烷组相比,两药合用组步入潜伏期和错误次数均无统计学差异(P>0.05)。结论 氟马西尼对异氟烷遗忘作用无明显影响,提示异氟烷遗忘作用与苯二氮?受体关系不大。更多还原

【Abstract】 Objective To investigate the effect of flumazenil on the isoflurane-induced amnesia and to reveal the relationship between benzodiazepine receptor and the mechanism of isoflurane-induced amnesia.Methods 48 mice were randomized into six groups.The effects of intraperitoneal injection of different doses of flumazenil(0.5,1.0 mg/kg) on the step-through latency and error times were observed in mice treated with isoflurane(0.2 ml/kg).Results Compared with the normal saline group,the step-through latency and error times had no statistical differences in the flumazenil groups(P <0.05);the isoflurance group and the isoflurance-flumazenil combination groups showed reduced step-through latency(P <0.05) and elevated error times(P <0.01 or P <0.05).Compared with the isoflurance group,no statistical differences in step-through latency and error times were observed between the isoflurance group and the isoflurance-flumazenil combination groups(P> 0.05).Conclusion Flumazenil has no significant influence on the isoflurance-induced amnesia,suggesting that the isoflurance-induced amnesia is not related to benzodiazepine receptor.更多还原