【摘要】 目的探讨邻苯二甲酸二乙基己基酯(DEHP)对大鼠睾丸细胞DNA的损伤和睾丸组织氧化应激的作用。方法雄性Wistar大鼠25只,随机分为5组,每组5只,通过灌胃的方法摄入DEHP,4个染毒组剂量分别为1 500、3 000、4 500、6 000 mg.kg-1.d-1,1个阴性对照组。用单细胞凝胶电泳技术检测大鼠睾丸细胞DNA的损伤,睾丸组织中活性氧(ROS)、丙二醛(MDA)的水平以及睾丸组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活力。结果各染毒组和阴性对照组大鼠睾丸细胞DNA损伤率分别为29.0%、71.0%、89.0%、95.0%和15.0%,各染毒组睾丸细胞DNA损伤程度与对照组比较,差异均有统计学意义(P<0.05,P<0.01),且大鼠睾丸细胞DNA损伤率与染毒剂量之间呈剂量-反应关系(r=0.930,P<0.01);各染毒组大鼠睾丸组织的ROS、MDA的水平均较阴性对照组显著升高(P<0.05,P<0.01),而GSH-Px、SOD的活力又较阴性对照组明显下降(P<0.05,P<0.01) DEHP可造成大鼠睾丸组织明显的氧化应激并引起睾丸细胞DNA的损伤。更多还原

【Abstract】 Objective To study the effect of di-(2-ethylhexyl) phthalate(DEHP) on DNA damage and oxidative stress in testicular tissue of rats. Methods Wistar male rats were divided into 5 groups,including 4 experimental groups,which were respectively exposed to 1 500,3 000,4 500 and 6 000 mg·kg-1·d-1 DEHP,and a control group.The degree of DNA damage in rat testicle cells was detected by single cell gel electrophoresis.Detected were the contents of reactive oxygen species(ROS),malondialdehyde(MDA) in testicular tissue,and the activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD) in testicular tissue. Results The rates of testicle cell DNA damage in each experimental groups and the control group were 29.0%,71.0%,89.0%,95.0%,and 15.0% respectively,showing DNA damage in all experimental groups were significantly higher than those in the control(P<0.05,P<0.01).There was a significant dose-response relation between the rates of testicle cell DNA damage and the doses of DEHP(r=0.930,P<0.01).The contents of ROS and MDA of 4 experimental groups increased significantly compared with those of the control(P<0.05,P<0.01).The activities of GSH-Px and SOD of 4 experimental groups reduced significantly compared with those of the control(P<0.05,P<0.01).Conclusion DEHP could cause oxidative stress and DNA damage in testis spermatogone of rats.更多还原