【摘要】 目的了解职业性三氯乙烯(TCE)药疹样皮炎(OMLDT)患者周围血T细胞抗原受体(TCR)Vβ亚家族基因谱系及其克隆性增殖情况。方法利用逆转录-聚合酶链反应(RT-PCR)分别检测10名健康人和5例OMLDT患者周围血中24个TCR Vβ基因的互补决定区3(CDR3)。PCR产物进一步经荧光标记和基因扫描分析CDR3长度而确定T细胞的克隆性。结果 10名健康人可检出所有24个TCR Vβ亚家族,5例OMLDT患者周围血仅检出1~6个Vβ亚家族阳性产物,平均表达(2.8±1.9)个Vβ亚家族,β亚家族检出数显著少于健康人(P<0.01)。OMLDT患者周围血中存在克隆性增殖T细胞,其中以Vβ16亚家族出现克隆性增殖的频率较高。结论 OMLDT患者周围血中TCR Vβ谱系发生改变,这可能是接触TCE后机体细胞免疫功能的异常变化。更多还原

【Abstract】 Objective To observe the diversity of TCR Vβ gene repertoire and clonal expansion in peripheral blood mononuclear cells from 10 health individuals and 5 patients with occupational medicamentose-like dermatitis due to trichloroethylene(OMLDT).Methods Complementarity determining region 3(CDR3) of 24 TCR Vβ genes were amplified using RT-PCR.The PCR products were further analyzed by genescan to evaluate the clonality of T cells.Results 24 Vβ subfamilies could be detected in 10 health individuals,while only 1~6 Vβ subfamilies were identified in 5 patients,showing that the detectable Vβ subfamilies in patients were significantly lower than those in the health individuals(P<0.01).Clonal expansion T cells in one or more Vβ subfamilies were found in 2 patients which were oligoclonal pattern.Conclusion Skewed distribution and clonal expansion of TCR Vβ subfamily T cells could be found in OMLDT patients.The changed repertoire of TCR Vβ T cells may be the result of the immune cytotoxicity induced by TCE.更多还原