【摘要】 为了研究中国西安地区人群HPA和HLA-Ⅰ分型的分布及多态性,采用PCR-SSP和PCR-SSO流式微磁珠技术检测西安地区375位血小板无偿捐献者HPA和HLA-Ⅰ分型并进行统计分析。结果发现,在HPA-1-HPA-17中,HPA-7-HPA-14、HPA-16和HPA-17只有-aa型,不具多态性;仅在HPA-3、HPA-15中检出-bb型。在16个表现型为HPA-5ab中有9个样本同时表达HPA-15ab,另7个样本表达-15bb,未见-15aa表型。此次检出的表型有HPA-1aa-17aa、HPA-1ab、-2ab、-3ab、-3bb、-4ab、-5ab、-6ab、-15ab和-15bb。在375人中可观察到16种HLA-A特异性,占该座位可检出表型特异性的76%(16/21),其中大于1%的有11种;观察到36种HLA-B特异性,占该座位可检出表型特异性的84%(36/43),其中大于1%的有23种,均涵盖在中国北方地区HLA-Ⅰ特异性常见型里。在375人中观察到HLA-A-B单体型264种,频率大于1%的有30种。结论:西安地区人群HPA和HLA-Ⅰ基因多态性基本符合中国北方地区分布情况,但具有西安地区特点。更多还原

【Abstract】 To study the allele frequencies and their polymorphism characteristics of human platelet antigen(HPA) and human leucocyte antigen-Ⅰ(HLA-Ⅰ) in Chinese xi’an population,the types of HPA and HLA-Ⅰ in 375 Chinese xi’an voluntary platelet donors were detected by PCR-SSP and PCR-SSO as well as flow cytometry with magnetic beads,and were analyzed.The results showed that there was no polymorphism in HPA-7-HPA-14,HPA-16 and HPA-17 which only expressed-aa type,the-bb type was only detected in HPA-3 and HPA-15,9 out of 16 samples for the HPA-5ab phenotype simultaneously expressed HPA-15ab,the other 7 samples expressed HPA-15bb,no HPA-15aa phenotype was observed.Phenotypes detected in this study were HPA-1aa-17aa,HPA-1ab,-2ab,-3ab,-3bb,-4ab,-5ab,-6ab,-15ab and-15bb.Among 375 cases,HLA-A specificity of 16 species was observed,which accounted for 76%(16/21) of detectable phenotype specificity in this locus,moreover,11 species showed frequency >1%;HLA-B specificity of 36 species was observed which accounted for 84%(36/43) of detectable phenotype specificity in this locus,moreover 23 species showed frequency>1%,these species were covered by common specific HLA-Ⅰ in northern China,264 species haplotype HLA-A-B were found in 375 cases,the frequency of 30 species was >1%.It is concluded that the gene frequency distribution of HPA and HLA-Ⅰ in Chinese Xi’an population is accordant with population of northern China on the whole,but it has its own characteristics.更多还原