【摘要】 本研究旨在探讨白介素6(IL-6)对人Th17细胞的调节作用。应用免疫磁珠分离正常人CD4+T细胞并培养。实验分为2组,实验组(IL-6+):CD4+T细胞(1×106/ml)经重组人IL-6(20 ng/ml)刺激4天;对照组(IL-6-):不经IL-6刺激。应用酶联免疫吸附试验(ELISA)检测上清中IL-17蛋白水平,流式细胞术(FCM)检测Th17细胞数量。结果发现,与对照组相比,经IL-6刺激后的CD4+T细胞上清中IL-17蛋白水平明显升高(337.05±189.09 pg/ml vs 15.07±12.70 pg/ml)(p<0.05);进一步FCM发现,IL-6刺激组Th17细胞数量明显高于对照组[(4.05±0.30)%vs(2.81±0.44)]%,(p<0.01)。结论:IL-6促进人Th17细胞的增殖。更多还原

【Abstract】 The aim of this study was to explore the regulatory function of interleukin-6(IL-6) on human Th17 cells.Human peripheral blood CD4+ T cells were purified from healthy donors by anti-CD4 monoclonal antibody(mAb) conjugated microbeads.The experiment was divided into 2 groups.Test group in which CD4+ T cells(1×106/ml) were stimulated by human recombined IL-6(20 ng/ml) for 4 days;control group in which CD4+ T cells did not stimulated by IL-6.The concentrations of IL-17 protein in the supernatants were assayed by enzyme-linked immunosorbentassay(ELISA),and quantity of Th17 cells were detected by flow cytometry.The results showed that as compared to control group,IL-17 protein level in the supernatants of CD4+ T cells significantly increased in IL-6 stimulated group:(337.05±189.09 pg/ml;vs 15.07±12.70 pg/ml)(p<0.05).Furthermore,the percentage of Th17 cells in cultures of CD4+ T cells stimulated by IL-6 was significantly higher than that in control group(4.05%±0.30% vs.2.81%±0.44%)(p< 0.01).It is concluded that IL-6 promotes the expansion of Th17 cells in vitro.更多还原