【摘要】 本研究旨在探讨lL-23及其它的IL-12家族成员在慢性特发性血小板减少性紫癜(ITP)的表达及其免疫调节功能。运用反转录实时PCR方法检测30例慢性ITP患者和15例正常对照者外周血单个核细胞(PBMNC)中IL-23p19、IL-12p35、IL-12p40、IL-27、IL-17 mRNA的表达水平,用ELISA方法检测血浆IL-23、IL-12、IL-17含量并分析其在慢性ITP中的表达规律及与T亚群的关系。结果表明,慢性ITP患者及正常人PBMNC均低水平表达IL-23p19、IL-12p35、IL-27、IL-12p40 mRNA;部分患者及正常人PBMNC微量表达IL-17 mRNA。IL-12p35、IL-27、IL-17mRNA的表达水平与正常对照相比无显著差异,IL-23p19、IL-12p40 mRNA的表达水平比正常对照明显减低(p<0.01)。慢性ITP患者血浆IL-12水平高于正常对照者(p<0.01),IL-23、IL-17水平与正常对照者相比无显著差异。结论:ITP患者体内T淋巴细胞异常主要与IL-12相关,可能与IL-23/IL-17调节轴无直接关系。更多还原

【Abstract】 The aim of this study was to investigate the expression and immunologic regulation function of interleukin-23 and its related cytokines in chronic idiopathic thrombocytopenic purpura(ITP) patients.Levels of cytokines in peripheral blood mononuclear cells(PBMNC) were detected by reverse-transcription real-time polymerase chain reaction in 30 patients with chronic ITP and 15 healthy volunteers.The quantity of IL-23,IL-12,IL-17 in serum was detected by enzyme-linked immunosorbent assay(ELISA).The results showed that low detectable mRNA levels of IL-23p19,IL-12p35,IL-27 and IL-12p40 were found in all patients and healthy persons.Trace of IL-17 mRNA were expressed in PBMNC of part of patients and normal controls.Levels of IL-12p35,IL-27,IL-17 mRNA between healthy persons and chronic ITP patients were not statistically different.Compared with normal controls,patients showed the lower expression levels of IL-23p19 and IL-12p40 mRNA(p<0.01).The IL-12 levels of chronic ITP patients were significantly higher than that of normal controls(p<0.01).The IL-23 and IL-17 levels of chronic ITP patients were same to that of normal controls.It is concluded that the imbalance of T cell subsets in ITP patients mainly associated with IL-12,but not with IL-23 and IL-17.更多还原