【摘要】 本研究探讨B细胞活化因子(BAFF)和B细胞淋巴瘤/白血病-2基因(BCL-2)mRNA在多发性骨髓瘤(MM)患者骨髓单个核细胞(BMMNC)中表达水平及BAFF和BCL-2对MM的发生、发展及预后的意义。收集40例MM患者及10例正常对照的骨髓标本,获取BMMNC;采用实时荧光定量PCR(real time PCR)的方法检测BMMNC中BAFF与BCL-2 mRNA的表达,并同步收取血浆,检测β2-MG分泌水平;按Durie-Salmon(D-S)分期标准对MM患者进行临床分期。结果表明,①BAFF与BCL-2 mRNA在MM组中表达升高,与对照组比较差异有统计学意义,治疗后平台期BAFF与BCL-2 mRNA表达水平明显减低(p<0.05);②复发/难治性多发性骨髓瘤患者BAFF与BCL-2 mRNA的表达水平较正常对照组及平台期患者明显升高(p<0.05),初发组与复发/难治组之间表达无显著差异。BAFF与BCL-2 mRNA表达与患者病程D-S分期和β2-MG水平有相关性。结论:MM患者BAFF与BCL-2mRNA表达水平升高,并且MM初治组、复发/难治组BAFF与BCL-2 mRNA表达水平均高于平台组,提示BAFF和BCL-2可能参与MM的发生、发展的病理机制;BAFF与BCL-2 mRNA表达水平与MM肿瘤负荷呈正相关,提示BAFF和BCL-2表达水平可以作为评判MM患者预后的新指标。更多还原

【Abstract】 This study was aimed to explore the expression of B cell-activating factor of TNF family(BAFF) and B cell lymyhoma/leukemia-2(BCL-2) in bone marrow mononuclear cells(BMMNC) of multiple myeloma(MM) and the significance of BAFF and BCL-2 for occurrence,development and prognosis of MM.The bone marrow of 40 cases of MM and 10 healthy persons was collected,the mononuclear cells(MNC) were isolated,the expression of BAFF and BCL-2 mRNA in BMMNC was detected by real-time PCR;the plasma was simultaneously collected and the β2-MG level was determined;the clinical staging of MM patients was performed according to Durie-Salmon(D-S) staging criterion.The results indicated that the expression level of BAFF and BCL-2 mRNA in MM patients increased,as compared with normal controls,the difference was statistical significant(p<0.05);the expression level of BAFF and BCL-2 mRNA in plateau stage after treatment obviously decreased.The expression level of BAFF and BCL-2 mRNA in relapsed/refractory MM patients was significanty higher than that in normal controls and patients reached plateau stage(p<0.05),there was no statistically significant difference between newly diagnosed and relapsed/refractory MM patients(p>0.05).The expression of BAFF and BCL-2 mRNA related with D-S staging and β2-MG level.It is concluded that the expression levels of BAFF and BCL-2 mRNA increase,morever the expression levels of BAFF and BCL-2 mRNA in newly diagnosed and relapsed/refractory MM patients are higher than those in patients reached plateau stage,which suggest the BAFF and BCL-2 may be involved in occurrence and development of MM;the relation of expression level of BAFF and BCL-2 mRNA to MM load is positive,which indicates the expression level of BAFF and BCL-2 mRNA may be a new indicator for evatuating the prognosis of MM patients.更多还原