【摘要】 本文旨在探讨人参皂苷Rg₁体外对金葡菌感染肺上皮细胞所致病变的作用及其可能的机制。实验采用体外大鼠原代肺上皮细胞感染模型。RT-PCR和Western blot方法观察肺细胞中integrinβ1,NF-κB和糖皮质激素受体（GR）等相关因子的表达。结果表明人参皂苷Rg₁可以明显抑制金葡菌感染后肺上皮细胞整合素integrinβ1的表达,下调炎性因子NF-κB,ICAM-1,TNF-α,IL-2和IL-6,上调GR的表达。人参皂苷Rg₁可以明显抑制金葡菌感染肺上皮细胞后的炎性因子,从而对肺脏起到一定的保护作用。更多还原

【Abstract】 The invasion of Staphylococcus aureus into respiratory epithelial cells and the followed inflammatory responses cause serious tissue damage.The aim of this study was to investigate the effects of ginsenoside Rg₁（Rg₁）on S.aureus infection in vitro and its action mechanism.An internalization model was constructed to determine the effect of Rg₁ on S.aureus invasion. The changes of expression of integrinβ1,NF-κB and glucocorticoid receptor were analyzed by Western blot.Expression of pro-inflammatory genes was validated using RT-PCR.The results demonstrated that Rg₁ treatment could reduce the invasion of S.aureus into rat pulmonary epithelial cells by down-regulating integrinβ1.Its anti-inflammatory action was exerted through reducing NF-κB and expressions of intercellular adhesion molecule-1（ICAM-1）,tumor necrosis factor-α（TNF-α）,interleukin-2 （IL-2）and interleukin-6（IL-6）.The increased expression of glucocorticoid receptor was involved in this regulation.The results suggested that Rg₁ could play a positive role in reducing S.aureus infections.Rg₁ could be used for the treatment of S.aureus infection,potentially.更多还原