【摘要】 红花是一种常用中药,红花注射液广泛地应用于临床治疗心脑血管疾病。本研究应用HPLC-DAD-ESI-MSn方法研究红花注射液中两种主要物质,羟基红花黄色素A和脱水红花黄色素B的稳定性及降解过程。考察了光照、温度、pH值对二者稳定性的影响。结果表明,羟基红花黄色素A和脱水红花黄色素B在温度大于60°C,pH≤3.0或>7.0时易发生降解且最终降解产物为对羟基桂皮酸,而光照对其影响不大。本研究应用LC-MS对其降解产物及降解过程进行推导,首次描述了两种物质的降解途径。此外ADP诱导的血小板凝集实验结果显示脱水红花黄色素B降解以后会大大降低红花注射液的抗凝活性。建议红花注射液生产及储存过程中应严格控制温度和pH值。更多还原

【Abstract】 Safflower is a popular Chinese medicinal plant and Safflower injection is extensively used for the clinical treatment of cerebrovascular and cardiovascular diseases. In this study, HPLC-DAD-ESI-MS n was utilized to study the stability and degradation of the two major but chemically unstable bioactive compounds hydroxysafflor yellow A and anhydrosafflor yellow B, in Safflower injection. The impact of light irradiation, temperature, and pH on the stability of these two compounds were studied. The results showed that hydroxysafflor yellow A and anhydrosafflor yellow B could degrade at high temperature (>60 °C) or extreme pHs (pH≤3.0 or >7.0), but not under light irradiation. The common degradation product was p-coumaric acid. Chemical structures of the other degradation products were characterized by LC-MS. Hypothetical degradation pathways were proposed. In addition, ADP-induced platelet aggregation tests showed that the degradation of anhydrosafflor yellow B could reduce the anticoagulation activities of Safflower injection. Our results suggest that temperature and pH are critically important for the preparation and storage of Safflower injection.更多还原