【摘要】 研究共4个试验:①按入氮温度分为-5、-30、-60、-90、-120℃5个组;②按鲜精液预处理方式分为未预稀释和预稀释2个组;③按解冻温度设37、50、70℃3个组;④按稀释后精子密度分为1.25×108、2.50×108、5.00×108spz/mL 3个组。结果表明:①不同入氮温度下精子冻后活力差异不显著,但随着入氮温度的降低,精子冻后活力呈升高趋势;②预稀释不能显著提高平衡后精子活力,但可以显著或极显著提高精子稀释后活力、冻后活力、VAP、VSL及VCL等运动指标;③3种解冻温度在精子冻后活力、VAP、VSL、VCL等4个冻后运动指标上没有显著差异,但是,随着解冻温度的上升,冻后活力呈上升趋势;④3种稀释后精子密度在猪精子冻后活力、VAP、VSL上没有显著差异,但随着密度的上升,冻后精子活力及VAP呈下降趋势,而且最高密度组精子的VCL显著慢于其它两组。总之,5种入氮温度中,以-120℃组精子的冻后活力最高;预稀释对于新鲜猪精液的冷冻保存很有必要;3种解冻温度中,70℃组精子冻后活力最高;而高密度不利于猪精子的冷冻保存。更多还原

【Abstract】 Four experiments were included in the present study.Five,two,three and three treatments were designed for experiment 1(plunging temperatures:-5,-30,-60,-90 and-120 ℃),experiment 2(fresh semen preprocessing: nonpre-extending and pre-extending),experiment 3(thawing temperatures: 37,50 ℃ and 70 ℃),and experiment 4(extended-spermatozoa concentration: 1.25×108,2.50×108 and 5.00×108 spz/mL) respectively.The results showed that(i) the differences of spermatozoa motility at different plunging temperatures were not significant,but motility of frozen-thawed spermatozoa revealed a tendency to improve with the drop of plunging temperature(ii) the preliminary dilution did not significantly increase motility of extended spermatozoa,but it made motility of equilibrated spermatozoa,motility,VAP,VSL and VCL of frozen-thawed spermatozoa significantly or very significantly higher,(iii) there were no significant differences among the three thawing temperatures in motility,VAP,VSL and VCL of frozen-thawed spermatozoa,however,the higher the thawing temperature was,the higher the motility of frozen-thawed spermatozoa was and(iv) three different concentrations of extended spermatozoa did not result in significantly different motility,VAP and VSL of frozen-thawed spermatozoa;nevertheless,the motility and VAP fell with the enhancement of extended-spermatozoa concentration,and the highest extended-spermatozoa concentration brought significantly lower VCL of frozen-thawed spermatozoa than the two other treatments.In conclusion,of the five plunging temperatures of the straw,the optimal one was-120 ℃,where the best sperm motility could be observed after thawing.And the preliminary dilution of fresh semen before extending was indispensable to cryopreservation of boar semen;besides,the highest motility of spermatozoa,thawed at 37,50 and 70 ℃ respectively,would be detected in the group 70 ℃,and moreover,high diluted-spermatozoa concentration could be disadvantageous to the cryopreservation of boar spermatozoa.更多还原