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利用AFLP标记技术评价甘蓝型油菜的遗传多样性和亲缘关系(英文)
Assessment of Genetic Diversity and Relationships among Brassica napus Using AFLP Markers
【摘要】 利用AFLP分子标记技术分析了25份甘蓝型油菜品种的遗传多样性。9对AFLP引物扩增出193条带,其中,73条呈多态性,多态性比率为38%。每对引物检测出的平均等位基因数为3.56,有效等位基因数为1.41。基因多样性、香农指数和遗传差异分别为0.25、0.62和0.39。在遗传相似系数0.66处,所有的甘蓝型油菜可分为3个类群,遗传相似系数表明,橄榄型油菜表现出丰富的遗传多样性。UPGMA聚类分析表明,品种间的亲缘关系与种质性状或来源关系不明显。
【Abstract】 AFLP markers were applied to assess genetic diversity and relationships among 25 B.napus cultivars.Nine AFLP primer combinations generated 193 fragments,of which 73 were polymorphic.The polymorphic rate was 38%.The average number of observed alleles per locus was 3.56,while the mean number of effective alleles was 1.41.Gene diversity,Shannon’s information index,and genetic differentiation were 0.25,0.62,and 0.39,respectively.At a genetic similarity coefficient of 0.66,all B.napus cultivars could be divided into three groups.A wide range of similarity coeffcient value suggested that the investigated B.napus germplasm collection represented a genetically diverse population.UPGMA cluster analysis revealed that no apparent clustering by traits or original locations.
- 【文献出处】 西南农业学报 ,Southwest China Journal of Agricultural Sciences , 编辑部邮箱 ,2011年04期
- 【分类号】S565.4
- 【被引频次】11
- 【下载频次】98