节点文献

利用AFLP标记技术评价甘蓝型油菜的遗传多样性和亲缘关系(英文)

Assessment of Genetic Diversity and Relationships among Brassica napus Using AFLP Markers

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 李丽黄先群Wanapu Chokchai雷尊国黄团李其义彭义黄贵民董颍苹

【Author】 LI Li1,2,HUANG Xian-qun1,,Wanapu Chokchai2,LEI Zun-guo1,HUANG Tuan1,LI Qi-yi3,PENG Yi3,HUANG Gui-min3,DONG Ying-ping1 (1.Guizhou Institute of Biotechnology,Guizhou Key Laboratory of Agricultural Biotechnology,Guizhou Guiyang 550006,China;2.School of Biotechnology,Suranaree University of Technology,Nakon Ratchasima 30000,Thailand;3.Guizhou Seed Management Station,Guizhou Guiyang 550002,China)

【机构】 贵州省生物技术研究所贵州省农业生物技术重点实验室泰国苏兰拉里理工大学农学院生物技术系贵州省种子管理站

【摘要】 利用AFLP分子标记技术分析了25份甘蓝型油菜品种的遗传多样性。9对AFLP引物扩增出193条带,其中,73条呈多态性,多态性比率为38%。每对引物检测出的平均等位基因数为3.56,有效等位基因数为1.41。基因多样性、香农指数和遗传差异分别为0.25、0.62和0.39。在遗传相似系数0.66处,所有的甘蓝型油菜可分为3个类群,遗传相似系数表明,橄榄型油菜表现出丰富的遗传多样性。UPGMA聚类分析表明,品种间的亲缘关系与种质性状或来源关系不明显。

【Abstract】 AFLP markers were applied to assess genetic diversity and relationships among 25 B.napus cultivars.Nine AFLP primer combinations generated 193 fragments,of which 73 were polymorphic.The polymorphic rate was 38%.The average number of observed alleles per locus was 3.56,while the mean number of effective alleles was 1.41.Gene diversity,Shannon’s information index,and genetic differentiation were 0.25,0.62,and 0.39,respectively.At a genetic similarity coefficient of 0.66,all B.napus cultivars could be divided into three groups.A wide range of similarity coeffcient value suggested that the investigated B.napus germplasm collection represented a genetically diverse population.UPGMA cluster analysis revealed that no apparent clustering by traits or original locations.

【关键词】 AFLP甘蓝型油菜遗传多样性
【Key words】 AFLPB.napusGenetic diversity
【基金】 贵州省科技攻关项目“甘蓝型油菜含油量基因的QTL分析”[黔科合NY字(2007)3006];贵州省科学技术基金项目“贵州马铃薯栽培品种的SRAP分析”[黔科合J字(2010)2084]~~
  • 【文献出处】 西南农业学报 ,Southwest China Journal of Agricultural Sciences , 编辑部邮箱 ,2011年04期
  • 【分类号】S565.4
  • 【被引频次】11
  • 【下载频次】98
节点文献中: 

本文链接的文献网络图示:

本文的引文网络