【摘要】 为了探讨表皮生长因子(EGF)对水牛早期胚胎体外发育及凋亡的影响,通过收集屠宰场卵巢卵母细胞进行体外成熟和体外受精,将假定的受精卵置于含不同浓度EGF(0,25,50和100 ng/mL)的培养液中培养,检查分裂率和囊胚发育率,用细胞凋亡试剂盒(Annexin-V-FluosStaining kit)试剂染色,统计囊胚细胞凋亡率和坏死率。结果表明:50 ng/mL EGF组的孵化囊胚率显著高于对照组(P<0.05),该组细胞凋亡率和坏死率显著低于对照组(P<0.05)。100 ng/mL EGF的卵裂率、囊胚率、D7囊胚率和孵化囊胚率显著低于对照组和其他试验组(P<0.05)。细胞凋亡率和坏死率显著高于其他各组(P<0.05)。提示:一定浓度的EGF可提高囊胚孵化率,并可抑制胚胎细胞的凋亡。更多还原

【Abstract】 To evaluate the influence of epidermal growth factor(EGF) on buffalo early embryo development and apoptosis in vitro,oocytes from buffalo ovaries at a slaughterhouse were matured and fertilized,and presumptive zygotes were cultured in the medium supplemented with EGF(0,25,50 or 100 ng/mL),then examined cleavage and blastocyst rate,respectively.After staining with Annexin V-Fluos Apoptosis Derection Kit(Annexin-V-Fluos Staining kit),apoptosis and necrosis of blastocyst under fluorescent microscope was examined.The result showed that the hatched rate in addition of 50 ng/mL EGF was significantly higher than that in controls(P<0.05),and apoptosis rate and necrosis rate were significantly lower than those in controls(P<0.05).While the cleavage rate,blastocyst rate,D7 blastocyst rate and the hatched rate in 100 ng/mL EGF in culture medium were lower than those in other groups,but the apoptosis rate and necrosis rate were higher.In conclusions,incubation of buffalo embryo in the presence of EGF improved the rate of hatched blastocyst and reduced the apoptosis of in vitro produced blastocysts.更多还原