【摘要】 本研究旨在对部分中国荷斯坦种公牛脊柱畸形综合征(Complex vertebral malformation,CVM)致病基因的携带状况进行筛查。应用错配PCR突变分析技术(PCR mismatch amplification mutation assay,PCR-MAMA)建立了针对CVM致病基因的特异性检测方法。利用PCR-MAMA法检测了154头荷斯坦种公牛,发现了24头CVM阳性个体,阳性率为15.58%。结果显示,应对中国荷斯坦种公牛进行全面的针对CVM的检测。更多还原

【Abstract】 This experiment was conducted to test some Chinese Holstein sires for complex vertebral malformation(CVM).In this study,a simple,rapid PCR mismatch amplification mutation assay(PCR-MAMA) was developed to detect the mutation allele.Out of 154 tested Holstein sires,24 sires(15.58%) were identified to be CVM carriers by PCR-MAMA.The results indicate that all the Chinese Holstein sires should be tested for CVM.更多还原