【摘要】 目的探讨吸烟对男性不育患者精液常规参数和精子DNA完整率的影响。方法调查310例男性不育患者,采用精液自动分析仪检测精液常规和吖啶橙荧光染色检测精子DNA完整率。结果不育吸烟组精子的精子活率、摆动性、精子畸形率与不育非吸烟组差异显著(P<0.05);a+b级精子百分率、平均移动角度、鞭打频率显著低于非吸烟组(P<0.01);不育吸烟组精子密度和形态低于不育非吸烟组,但无统计学意义。不育吸烟组精子DNA完整率异常例数显著高于不育非吸烟组(χ2=4.596,P<0.05)。结论吸烟可以影响精液常规参数和精子DNA完整率。更多还原

【Abstract】 Objective To study the influence of cigarette smoking on the routine semen analysis and sperm DNA integrity. Methods The cigarette smoking status of 310 male patients with infertility was surveyed.The semen routine was analyzed by automatic semen analyzer and human sperm DNA integrity was detected by fluorescent staining of acridine orange.Results The sperm motility,the swing of sperms,and the percent of abnormal sperms of the smoker group were significantly lower than those of the nonsmoker group(P<0.05).The percent of a+b sperms,the average moving angle,and beat frequency of the smoker group were significantly lower than those of the nonsmoker group(P<0.01).The sperm density and morphology of the smoker group were lower than those of the nonsmoker group but without significant difference(P<0.05).The abnormal sperm DNA integrity of the smoker group was significantly higher than that of the nonsmoker group(χ2=4.596,P<0.05.) Conclusions Cigarette smoking affects routine semen parameters and sperm DNA integrity.更多还原