【摘要】 甘油脱氢酶(GDH)可以催化甘油生成二羟基丙酮.为了进一步探索GDH的活性结构中心,提高GDH的活性,用Mg2+、Mn2+、Ba2+、Ca2+、Zn2+、Cu2+、Sr2+、Ni2+、Cd2+和Co2+等10种金属离子对GDH进行活性中心金属离子置换修饰,筛选出具有修饰效果的离子为Mg2+、Mn2+和Ba2+,修饰后其最大酶活力分别是初始酶活力的3.4,2.0和4.7倍.通过均匀设计对3种金属离子的修饰条件进行了优化,优化后测定的酶活力分别是未修饰GDH酶活力值3.17 U/mL的14.9,11.3和12.4倍,极大地提高了GDH的酶活力.更多还原

【Abstract】 Glycerol dehydrogenase(GDH) can catalyze the interconversion of glycerol and dihydroxyacetone.In order to improve the activity and explore the active central site of GDH,10 kinds of metal ions such as Mg2+,Mn2+,Ba2+,Ca2+,Zn2+,Cu2+,Sr2+,Ni2+,Cd2+and Co2+ were used to do chemical modification on GDH.The results approved that the activity of GDH was significantly increased by Mg2+,Mn2+ and Ba2+ while the special activity of GDH was 3.4,2.0 and 4.7 folds respectively of the control′s after modification.Meanwhile,the activity effects of Mg2+,Ba2+ and Mn2+ on GDH showed that the GDH was successfully modificated by Mg2+,Ba2+ and Mn2+.The modificated condition was optimized with the uniform design.Compared to the original activity of GDH 3.17 U/mL,the activity of GDH modified by Mg2+,Ba2+ and Mn2+ was improved separately as 14.9,11.3 and 12.4 folds after optimization.更多还原