【摘要】 [目的]研究大鼠肺动脉高压后基质金属蛋白酶(MMPs)和金属蛋白酶组织抑制因子(TIMPs)在左右心室中表达与在心室重构中的作用。[方法]60只SD随机等分为5组,每组12只,1个对照组和4个肺动脉高压实验组(PAH组),分别于4个时间点(MCT注射后第1,2,3,4周)取材。将MCT注射后第1,2,3,4周的4个肺动脉高压实验组(PAH组)分别简称PAH1、PAH2、PAH3和PAH4实验组。采用酶谱法、免疫印迹(western Blotting)、逆转录-聚合酶链反应(RT-PCR),探讨基质金属蛋白酶-2,9(MMP-2,9)蛋白和mRNA的变化规律。[结果](1)右心室MMP-2,9 mRNA在肺动脉高压后1周增加达高峰,以后逐渐下降但到4周时比对照组仍然要高,并且差异有统计学意义(P﹤0.01)。TIMP-1 mRNA在肺动脉高压后从第1周就开始减少,一直到第4周仍然减少,与对照级比较,差异有统计学意义(P﹤0.01)。(2)左心室MMP-2,9蛋白水平在大鼠肺动脉高压后活性稍增强,表达稍增加,而TIMP-1蛋白含量也稍减少,与对照组相比没有统计学差异,并不导致左心室重构。[结论]MMP-2,9蛋白活性与表达在大鼠肺动脉高压右心室心肌中增强,而TIMP-1蛋白活性与表达减少,它们可能与右心室心肌重塑有关。左心室并不重构。更多还原

【Abstract】 [Objective]To explore the role of the matrix metalloproteinases(MMPs)and tissue inhibitor of metalloproteinase(TIMPs)in the left and right ventricle expression and remodeling on rats with Pulmonary hypertension.[Methods]60 SD rats were randomly divided into 5groups.12 SD rats were in each group.There were one control group and 4 experimental pulmonary hypertension(PAH)groups.We took samples at 4 time Points(MCT were injected at the 1,2,3 and 4 week).MCT would be injected at 1,2,3 and 4 week after experimental pulmonary hypertension(PAH),the corresponding experimental groups were regarded as PAH1,PAH2,PAH3 and PAH4 experimental groups.The activity of MMPs was measured by zymography.The protein and mRNA of MMP-2,-9 and TIMP-1 were determined by Western-Blot an d RT-PCR.[Results](1)MMP-2 and 9 mRNA reached peak at 1 week after Pulmonary hypertension,and gradually declined to 4 weeks,but compared with the control level.The experimental group was still higher,there were significant differences significance(P﹤0.01).TIMP-1 mRNA reduced in the experimental group from the first to the fourth week.Compared with the control level,there were significant differences significance(P﹤0.01).(2)In the left ventricle,MMP-2 and 9 Protein activity slightly increased in rat Pulmonary hypertension,the expression slightly increased,while TIMP-1 Protein content was also slightly reduced.But there was no significantly difference with the control group,and they could not lead to left ventricular remodeling.[Conclusion]MMP-2,9 Protein activity and its expression increased in rat right ventricle with Pulmonary hypertension.But TIMP-1 Protein activity and expression decreased in rat right ventricle with Pulmonary hypertension.They may be related to right myocardial remodeling.Not left ventricular remodeling.更多还原