【摘要】 目的制备重组人睫状神经营养因子(recombinant human ciliary neurotrophic factor,rhCNTF)脂质体,提高rhCNTF穿透血脑屏障能力。方法采用薄膜超声法制备rhCNTF脂质体,测定包封率。分别用rhCNTF原液、rhCNTF脂质体及生理盐水尾静脉注射Balb/C小鼠,0,2,6,12,24 h后测小鼠脑组织中rhCNTF含量。结果制得的rhCNTF脂质体形态圆整,平均直径为85.0 nm,包封率为93.14%。用ELISA法测定小鼠脑组织中rhCNTF的含量,rhCNTF原液组和rhCNTF脂质体组存在显著差异。结论制备的rhCNTF脂质体能提高rhCNTF穿越血脑屏障的能力,为rhCNTF的进一步应用和研究提供了依据。更多还原

【Abstract】 OBJECTIVE To enhance the penetration ability to cross the blood-brain barrier efficiently of recombinant human ciliary reurotrophic factor(rhCNTF) by encapsulated into liposome.METHODS rhCNTF liposomes were prepared by thin film dispersion-sonication method.Encapsulation efficiency was detected by HPLC.Mice were killed 0,2,6,12 and 24 h after i.v.administration of rhCNTF solution,rhCNTF liposomes or saline,and then the content of rhCNTF in brain tissues was analyzed by ELISA.RESULTS rhCNTF liposomes were regular in its morphology with a mean diameter of 85.0 nm.The entrapment efficiency of rhCNTF was 93.14%.rhCNTF in mice brain tissue were different between rhCNTF solution group and rhCNTF liposomes group.CONCLUSION Liposome-encapsulated rhCNTF could efficiently cross the blood brain barrier,which provides a basis for further application and research of rhCNTF.更多还原