【摘要】 背景:高浓度葡萄糖培养条件下,细菌在生物材料表面有较强的生物膜形成能力。目的:观察血糖升高对表皮葡萄球菌在动物体内清除能力及植入性生物材料上细菌生物膜形成的影响。方法:注射链脲佐菌素建立高血糖树鼩模型(血糖≥11.1mmol/L),采用生物膜形成阳性与阴性表皮葡萄球菌感染高血糖与正常对照树鼩,并同时在动物股静脉内植入PVC导管。结果与结论:感染生物膜形成阳性的表皮葡萄球菌株后,血糖≥11.1mmol/L树鼩血液、心脏、肝脏、肾脏、胰腺的细菌感染率及菌落计数较正常对照组高(P<0.05);扫描电镜观察血糖≥11.1mmol/L组植入生物材料上有明显的生物膜形成。感染生物膜形成阴性表皮葡萄球菌株后无论血糖高低,均未观察到生物膜形成。表明血糖升高不仅使植入生物材料树鼩的细菌清除能力下降,同时可诱导细菌在植入生物材料表面形成明显的生物膜。更多还原

【Abstract】 BACKGROUND:High-concentration glucose can induce the formation of biofilms in Staphylococcus epidermidis.OBJECTIVE:To investigate the effect of high-concentration glucose on eliminating ability and adhesion of Staphylococcus epidermidis in Tupaia belangeri.METHODS:Tupaia belangeri models of hyperglycaemia(≥11.1 mmoL/L) were induced by intraperitoneal injection of Streptozocin.PVC catheters were inserted into the left femoral vein,and Staphylococcus epidermidis with or without formation of biofilms was inoculated into the catheters.RESULTS AND CONCLUSION:Following transfection of Staphylococcus epidermidis with formation of biofilms,there were higher rates of bacterial infection as well as higher bacterium colony number in the serum,heart,liver,kidney and pancreas of Tupai belangeri in hyperglycemia group(≥11.1 mmoL/L) as compared with the control group(P < 0.05).Detected by scanning electronic microscope,biofilm formation was remarkable in hyperglycemia group(≥11.1 mmoL/L)(P < 0.05).However,there was no formation of biofilms in hyperglycemia or control groups following transfection of Staphylococcus epidermidis without formation of biofilms.Hyperglycemia can induce the decreasing ability of eliminating bacteria and the increasing formation of biofilms on the surface of biomaterials transfected with Staphylococcus epidermidis.更多还原