【摘要】 背景:人类免疫缺陷病毒能够结合人胸腺的细胞CD4受体,从而导致CD4细胞活性降低。目的:观察人胚胎胸腺组织原代培养与人类免疫缺陷病毒相互作用关系。方法:取人胎胸腺组织进行原代细胞培养,取胸腺的细胞与人类免疫缺陷病毒1ⅢB混合培养设为实验组,设置不加人类免疫缺陷病毒培养的胸腺的细胞为对照组。结果与结论:透射电镜观察显示,培养40h,人类免疫缺陷病毒诱导的胸腺的细胞内即可发现大量艾滋病病毒颗粒。MTT法和免疫组织化学染色检测结果显示,培养40h~22d,HIV诱导胸腺的细胞吸光度值均显著低于对照组(r=0.9733,P<0.05),人类免疫缺陷病毒诱导胸腺的细胞膜上和细胞浆Fas-L阳性表达率均升高(P<0.05)。说明人类免疫缺陷病毒可致原代培养的人胚胎胸腺的细胞活性降低。更多还原

【Abstract】 BACKGROUND:Human immunodeficiency virus binds to CD4 receptors in human thymocytes,and thus causes activity decreasing in CD4 cells.OBJECTIVE:To explore the relationship between human embryo thymocytes and human immunodeficiency virus.METHODS:Primary cultured human embryo thymocytes were divided into experiment group and control group.Experimental thymocytes were mixed cultured with human immunodeficiency virus 1 Ⅲ B.Control thymocytes were cultured without human immunodeficiency virus.RESULTS AND CONCLUSION:According to transmission electron microscopy,massive human immunodeficiency virus particles emerged in thymocytes treated with human immunodeficiency virus at the 40th hour after cultivation.According to MTT assay and immunohistochemical staining,the absorbance in thymocytes treated with human immunodeficiency virus was significantly lower than that in the control thymocytes at the 40th hour to the 2nd day after cultivation(r=0.9733,P < 0.05).The Fas-L positive rate of the experiment thymocytes treated with human immunodeficiency virus increased on cell membranes and in cytoplasm(P < 0.05).These findings indicate that human immunodeficiency virus decreases the cell activity in primary cultured human embryo thymocytes.更多还原