【摘要】 背景:对常染色体STR基因座的多态性的研究可为法医学亲权鉴定提供基础数据。目的:探索辽宁锡伯族D16S539,THO1,D13S3173个常染色体基因座的遗传多态性,建立锡伯族群体的遗传学基础数据。方法:采集辽宁省沈阳市新城子区黄家乡锡伯族中小学的150名中小学生口腔黏膜细胞,Chelex100法提取DNA,进行荧光标记PCR扩增,产物在Li-COR4300基因分析仪上进行电泳,E-seq分析软件计算扩增产物片段相对大小,进行基因型分型。调查辽宁地区锡伯族群体3个STR基因座等位基因频率,进行遗传多态性分析。结果与结论:辽宁锡伯族群体中3个STR基因座具有遗传多态性,其基因型分布符合Hardy-Weinberg平衡定律。辽宁锡伯族群体中3个STR基因座的杂合度分布在0.769~0.810;个人识别力分布在0.824~0.929,累积个人识别能力为0.999;多态信息量分布在0.650~0.790;非父排除率分布在0.565~0.790,累积非父排除率为0.979。说明辽宁锡伯族群体3个常染色体STR基因座有较高的非父排除率和个体识别能力,可为法医学亲子鉴定和个体识别及移植配型等遗传学研究提供依据。更多还原

【Abstract】 BACKGROUND: Studying the gene polymorphism of autosome short tandem repeat (STR) gene loci can provide basic data for forensic paternal power identification. OBJECTIVE: To explore the genetic polymorphism of three STR loci, D16S539, THO1, D13S317, in Liaoning Xibo population of China and establish the genetic basic data of Xibo population. METHODS: Buccal samples were obtained from 150 primary and middle school students of Xibo population living in Liaoning province and PCR amplification reactions of each STR loci were performed in fluorescence-based reaction using a 2720 Thermal cycler. The amplified products were detected in a Li-COR 4300 DNA Analyzer. Relative fragment size of amplified products was calculated through the use of E-seq Analysis software and genotyping was performed. Allele frequency of three STR genetic loci of Xibo population living in Liaoning province was investigated and genetic polymorphism analysis was performed. RESULTS AND CONCLUSION: All the three loci met the Hardy-Weinberg equilibrium. Heterozygosis degree of these three loci was distributed between 0.769-0.810. Individual discriminative power was distributed between 0.824-0.929. Cumulative individual discriminative power was 0.999. Polymorphism information was distributed between 0.650-0.790. Non-parental eliminant rate was distributed between 0.565-0.790. Cumulative non-parental eliminant rate was 0.979. The three STR loci used in this study show high non-parental eliminant rate and individual discriminative power in Xibo population and can be applied to population study and forensic practice.更多还原