【摘要】 背景:大部分研究显示,太极拳运动有望通过改善机体免疫功能而延缓衰老,但其机制不明。目的:观察太极拳运动后外周血白细胞热休克蛋白70mRNA的表达情况。方法:在健康中老年女性学练太极拳前、练习3个月和6个月时对其外周血白细胞热休克蛋白70mRNA表达进行实时定量PCR检测。结果与结论:经过3个月的太极拳运动干预,中老年妇女外周血白细胞热休克蛋白70mRNA的表达量与干预前比略增加,但没有明显差异,但干预6个月后表达量下降明显,与干预前和干预3个月的表达量相比差异均有显著性意义。结果初步表明热休克蛋白mRNA表达的改变反映了太极拳健身的效果,而热休克蛋白的分子伴侣作用是太极拳健身效果的可能机制。更多还原

【Abstract】 BACKGROUND:Several studies have demonstrated that Taiji-quan exercise is promosing to improve organism’s immune function and then postpone aging,but the underlying mechanisms remain poorly understood.OBJECTIVE:To investigate the heat shock protein 70(HSP70) expression in peripheral white blood cells after Taiji-quan exercise.METHODS:The HSP70 mRNA expression was detected by real-time quantitative PCR in peripheral white blood cells of healthy middle-aged and elderly women before,3 and 6 months after Taiji-Quan exercise.RESULTS AND CONCLUSION:After 3 months of intensive Taiji-Quan exercise,HSP70 mRNA expression was slightly,but not significantly,increased,compared with before Taiji-Quan exercise.After 6 months of Taiji-Quan exercise,HSP70 mRNA expression was significantly decreased,compared with before exercise and after 3 months of Taiji-Quan exercise.These results suggest that HSP70 mRNA expression can reflect the health benefits of 48-style Taiji-Quan exercise in middle-aged and elderly women and HSP 70 may be the potentioal molecular biological mechanism.更多还原