【摘要】 背景:关于雌激素对骨髓基质干细胞作用的报道尚不多。目的:观察乙烯雌酚对兔骨髓基质干细胞成骨分化的影响。方法:体外培养骨髓基质干细胞,用0,10-7,10-6,10-5 mol/L 浓度乙烯雌酚干预,并设地塞米松 10-8mol/L、β-甘油磷酸钠10mmol/L、维生素C50 mg/L为阳性对照。结果与结论:乙烯雌酚干预培养 24,48,72 h,10-6mol/L 组显著促进了骨髓基质干细胞增殖(P< 0.01)。干预 48h10-5mol/L组显著抑制细胞增殖,干预72h 10-7mol/L组显著抑制细胞增殖(P<0.01)。10-7mol/L组乙烯雌酚干预25d 后开始出现钙化结节;10-7,10-6mol/L组干预 14,21d碱性磷酸酶活性显著增加。证实乙烯雌酚能促进兔骨髓基质干细胞的成骨分化。更多还原

【Abstract】 BACKGROUND: There are fewer reports about estrogen effects on bone marrow stromal cells (BMSCs). OBJECTIVE: To study the effect of diethylstilbestrol on the osteogenic differentiation of rabbit BMSCs. METHODS: Rabbit BMSCs cultured in vitro were intervened with 0, 10-7, 10-6, 10-5 mol/L diethylstilbestrol, and BMSCs cultured with dexamethasone 10-8 mol/L, β-sodium glycerophosphate 10 mmol/L, and vitamin C 50 mg/L were used as positive controls. RESULTS AND CONCLUSION: 10-6 mol/L diethylstilbestrol significantly improved the proliferative ability of BMSCs at 24, 48, and 72 hours after intervention (P < 0.01). 10-5 mol/L diethylstilbestrol significantly inhibited the proliferation of BMSCs at 48 hours after intervention as well as 10-7 mol/L diethylstilbestrol at 72 hours (P < 0.01). Mineralized nodular structures formed at 25 days after intervention with 10-7 mol/L diethylstilbestrol. Alkaline phosphatase activities were remarkably increased at 14 and 21 days after intervention with 10-7, 10-6 mol/L diethylstilbestrol. It has been proved that diethylstilbestrol has an enhancing effect on the osteogenic differentiation of rabbit BMSCs.更多还原