【摘要】 背景:骨骼肌含有被称为"分子伴侣"的小热休克蛋白αB-晶状体蛋白,有可能在肌肉运动中具有重要的生理功能。目的:观察离心运动后骨骼肌细胞αB-晶状体蛋白基因表达。方法:将成年雄性Wistar大鼠随机分为安静对照组和运动后即刻组,运动后24h组。安静对照组正常喂养,两个运动组进行一次性大负荷离心运动,分别取对照组腓肠肌和运动组运动后0和24h的腓肠肌,使用冰冻切片原位杂交法检测αB-晶状体蛋白基因表达情况。结果与结论:骨骼肌细胞αB-晶状体蛋白基因在对照组呈低水平表达,在运动后即刻和运动后24h组均呈较高表达(P<0.05),αB-晶状体蛋白基因表达杂交信号主要位于肌细胞膜下。结果证实,大强度离心运动可诱导骨骼肌细胞αB-晶状体蛋白基因表达增高。更多还原

【Abstract】 BACKGROUND:Skeletal muscle is called "molecular chaperones" the small heat shock protein αB-crystallin protein in the exercise training may have important physiological functions. OBJECTIVE:To examine the mRNA expression of αB-crystallin in skeletal muscle after eccentric exercise. METHODS:Adult male Wistar rats were randomly divided into control group, immediately after exercise group and 24 hours after exercise group. Control group received normal feeding. Exercise groups experienced once high-intensity eccentric exercise. After exercise, gastrocnemius muscle from excise groups at 0 and 24 hours after excise and those from control group were removed. The gastrocnemius muscles were processed for cryosection in situ hybridization to measure αB-crystallin mRNA expression. RESULTS AND CONCLUSION:Compared with control group which had a weak level of labeling, the hybridization signal of αB-crystallin mRNA was obviously enhanced near muscle cell membrane in immediately after exercise group and 24 hours after exercise group (P < 0.05). Results suggest that αB-crystallin mRNA expression was up-regulated after high-intensity eccentric exercise.更多还原