【摘要】 背景:对罗汉果的遗传毒性进行研究,可为其安全使用提供实验依据。目的:观察罗汉果水提液对雄性小鼠骨髓细胞微核率和附睾精子畸形率的影响,了解其是否有遗传毒性。方法:按罗汉果水提液最大使用剂量(3g/mL)和最大灌胃容量(20mL/kg)灌胃小鼠,观察罗汉果水提液的急性毒性。将雄性昆明小鼠随机分为5组,分别灌胃给予30,15,7.5g/kg的罗汉果水煎液、蒸馏水,连续5d;或腹腔注射40mg/kg环磷酰胺。于灌胃第5天,采用骨髓嗜多染红细胞微核试验计算小鼠的骨髓微核率。于首次灌胃后第35天,观察小鼠精子畸形率。结果与结论:罗汉果水提液对昆明小鼠的经口急性毒性最大耐受剂量大于120g/kg。罗汉果水提液30,15,7.5g/kg灌胃后,小鼠的骨髓微核率、精子畸形率与正常小鼠无差异(P>0.05),均明显低于环磷酰胺诱发的骨髓微核率和精子畸形率(P<0.05)。说明罗汉果水提液对成年雄性小鼠无明显遗传毒性。更多还原

【Abstract】 BACKGROUND:The investigation into the genotoxicity of grosvenor momordica fruit will provide experimental evidence for its safe application. OBJECTIVE:To investigate effects of aqueous extracts from grosvenor momordica fruits on micronuclear rates of bone marrow cells and teratosperm rate of epididymis in male mice to identify whether the water extracts have genetic and reproductive toxicity. METHODS:Male Kunming mice intragastrically perfused with the maximum using concentration (3 g/mL) and maximum perfusion volume (20 mL/kg) to observe acute toxicity of aqueous extracts from grosvenor momordica fruits. The rats were randomly divided into five groups, respectively perfused with 30, 15, 7.5 g/kg aqueous extracts, distilled water for 5 consecutive days and intraperitoneally injected with cyclophosphamide (40 mg/kg). Bone marrow micronuclear rates were tested using bone marrow cell micronucleus test at the fifth day of perfusion. Teratosperm rate was observed at the 35th day after the first perfusion. RESULTS AND CONCLUSION: The oral maximum tolerated dose of aqueous extracts of grosvenor momordica fruits for Kunming mouse was 120 g/kg. After gastric administration of aqueous extracts at 30, 15, and 7.5 g/kg, there was no obvious difference in micronuclear rates or teratosperm rate compared with normal mice (P > 0.05), which were significantly lower than cyclophosphamide group (P < 0.05). Results demonstrated that aqueous extracts from grosvenor momordica fruits have no evident genetic toxicity to adult male mice.更多还原