【摘要】 背景:通过调节神经干细胞分化过程中的tau蛋白磷酸化水平可提高神经干细胞移植治疗阿尔茨海默病的效果。目的:观察β-淀粉样蛋白25～35和人参皂苷Rb1对神经干细胞分化过程中tau蛋白磷酸化水平的影响。方法:分离、培养新生大鼠海马神经干细胞,诱导第3代神经干细胞分化1周后,分别采用免疫荧光细胞化学和Western-blot检测Tau[pS396]、Tau[pS262]及GSK-3β[pTyr279,216]表达情况。结果与结论:正常神经干细胞分化过程中有Tau[pS396]和Tau[pS262]的表达,β-淀粉样蛋白25～35可以使Tau[pS396]、Tau[pS262]和GSK-3β[pT279/216]表达上调,而人参皂苷Rb1可以逆转β-淀粉样蛋白25～35的作用。提示β-淀粉样蛋白25～35和人参皂苷Rb1通过调节GSK-3β的活性对tau蛋白的磷酸化水平产生调控作用。更多还原

【Abstract】 BACKGROUND:Currently,neural stem cells(NSCs) transplantation effect on the treatment of Alzheimer’s disease is improved by adjusting tau protein phosphorylation level during the differentiation of NSCs.OBJECTIVE:To explore the effect of GinsenosideRb1 and beta-amyloid 25-35(Aβ25-35) on tau phosphorylation after NSCs are transformed into neurons.METHODS:NSCs were isolated and cultured from rat hippocampus.NSCs of the third passage were induced towards neurons after one week;the expression of Tau[pS396],Tau[pS262] and GSK-3β[pTyr279,216] were tested by the immunofluorescent cytochemical staining and western-blot.RESULTS AND CONCLUSION:The expression of Tau[pS396],Tau[pS262] and GSK-3β[pTyr279,216] was found in the process of NSCs differentiation.Aβ25-35 could enhance their expression;moreover Ginsenoside Rb1 could reverse the effect of Aβ25-35.The results indicated that there was a certain level of phosphorylation in tau protein during NSCs differentiation.Aβ25-35 and GinsenosideRb1 could regulate the level of tau phosphorylation.The mechanism of its action is by adjusting GSK-3β activity.更多还原