【摘要】 背景:肝储脂细胞产生胶原是导致肝硬化的直接原因,调控肝储脂细胞产生胶原的能力即可以防治肝硬化。目的:观察人参皂苷Rd对体外培养肝储脂细胞Ⅰ,Ⅲ型胶原表达的影响。方法:体外培养肝储脂细胞,随机分为对照组和实验组,实验组用100mg/L人参皂苷Rd进行培养干扰,用免疫组织化学、免疫荧光技术检测Ⅰ,Ⅲ型胶原基因的表达。结果与结论:实验组比对照组,Ⅰ,Ⅲ型胶原阳性产物吸光度和面积比值明显降低(P<0.05),说明人参皂苷Rd对体外培养肝储脂细胞Ⅰ,Ⅲ型胶原的表达有明显调节作用,影响肝储脂细胞产生胶原纤维。更多还原

【Abstract】 BACKGROUND:Collagens produced by liver fat-storing cells are the directly reasons for cirrhosis,thus,regulating capacity of fat-storing cells producing collagens can prevent cirrhosis.OBJECTIVE:To explore effect of ginsenoside Rd on expression of type Ⅰ and Ⅲ collagens in liver fat-storing cells in vitro.METHODS:The liver fat-storing cells were cultured in vitro and interfered with ginsenoside Rd.The expression of type Ⅰ and Ⅲ collagens was detected by immunohistochemistry and immunofluorescent.RESULTS AND CONCLUSION:Compared with the control group,the positive/negative-area and the absorbance of the experimental group were obviously decreased(P < 0.05),suggesting that ginsenoside Rd regulates the expression of type Ⅰ and Ⅲ collagens in liver fat-storing cells in vitro,which affects liver fat-storing cells producing collagens.更多还原