【摘要】 背景:吉兰-巴雷综合征患者血液中存在与发病有关的抗体、补体和细胞因子,以蛋白质组学技术分离鉴定这些标志蛋白,可为寻找新的药物靶标提供依据。目的:以蛋白质组技术比较吉兰-巴雷综合征患者与正常对照组血清的差异表达蛋白。方法:采集确诊的吉兰-巴雷综合征患者和正常者血清各30例,提取血清蛋白质以固相pH梯度等电聚焦为第一向,SDS-PAGE垂直电泳为第二向进行双向电泳,图象分析软件Imagemaster 2D分析电泳图谱,MALDI-TOF/TOF串联质谱鉴定差异表达蛋白。结果与结论:在吉兰-巴雷综合征患者与正常者中24种蛋白质的表达量显著不同,其中α-2-巨球蛋白,血浆铜蓝蛋白,血清淀粉样P物质,丛生蛋白,抗糜蛋白酶,触珠蛋白,血红素蛋白,α-1-抗胰蛋白酶,血清转铁蛋白等9种蛋白质被鉴定为急性期反应蛋白。结果说明吉兰-巴雷综合征患者血清中急性期反应蛋白表达量发生了明显变化,加深了对吉兰-巴雷综合征发病分子机制的理解。更多还原

【Abstract】 BACKGROUND:Antibody,complement and cytokine related to the pathogensis of Guillain-Barré syndrome(GBS) existed in blood of GBS patients.Identification of these marker proteins can provide evidence for searching new drug targets.OBJECTIVE:To compare differential proteins expression between GBS patients and normal controls by proteomics technology.METHODS:The proteins extracted from GBS patients and normal people were run immobilized pH gradient(IPG) isoelectric focusing electrophoresis as the first dimension,and then run vertical SDS-PAGE as the second dimension.The maps were visualized by silver staining or colloidal coomassive blue and analyzed with ImageMaster 2D Elite software.The proteins of interest were in-gel digested and identified using MALDI-TOF/TOF tandem mass spectrometry.RESULTS AND CONCLUSION:All 24 protein spots were differentially expressed as compared with age-matched control serum,and 9 proteins out of which were members of the acute phase protein,which were identified as α-2-macroglobulin,ceruloplasmi,serum amyloid P-component,Clusterin,α-1-antichymotrypsin,Haptoglobin,Hemopexin,α-1-antitrypsin,Serotransferrin.We got a number of related-proteins of GBS.Some of the proteins are members of the acute phase protein,which are quite useful for discovering the molecular mechanisms of GBS.更多还原