【摘要】 目的探讨脑脊液早期分泌抗原靶-6（ESAT-6）和干扰素-γ表达水平变化对结核性脑膜炎的临床诊断价值。方法采用酶联免疫吸附试验分别检测结核性脑膜炎（21例）、非结核性脑膜炎颅内感染（28例）和正常对照者（32例）脑脊液ESAT-6和干扰素-γ表达水平。结果结核性脑膜炎组患者脑脊液ESAT-6和干扰素-γ平均表达水平分别为4.46 pg/ml[（2.20～10.55）pg/ml]和34.86 ng/L[（25.62～241.71）ng/L],均明显高于非结核性脑膜炎颅内感染组[（1.18±0.49）pg/ml、（12.00±3.37）ng/L]和正常对照组[（1.05±0.47）pg/ml、（14.58±3.46）ng/L],差异具有统计学意义（H=35.695,P=0.000;H=31.560,P=0.000）。非结核性脑膜炎颅内感染组与正常对照组之间差异无统计学意义（t=1.226,P=0.226;t=0.060,P=0.952）。Spearman秩相关分析显示,结核性脑膜炎组患者ESAT-6水平与干扰素-γ水平呈正相关(r_?=1.000,P=0.000)。结论结核性脑膜炎患者脑脊液ESAT-6和干扰素-γ表达水平明显高于非结核性脑膜炎颅内感染和正常对照者,检测这两项实验室指标的变化可协助结核脑膜炎的诊断,月.两项指标之间具有一定相关性。更多还原

【Abstract】 Objective To explore the value of early secretory antigenic target-6(ESAT-6) and interferon-γ(IFN-γ) in cerebrospinal fluid(CSF) for the diagnosis of tuberculous meningitis(TBM). Methods Eighty-one patients were examined in our hospital or outpatient clinic.Referenced to the diagnostic criteria of TBM,patients were divided into 3 groups:21 cases were patients with TBM;28 were patients with intracranial infection of non-TBM;and 32 controls without nervous system disease.CSF sample was collected by standard lumbar puncture and was used for detection of ESAT-6 and IFN-γwith enzyme-linked immunosorbent assay(ELISA).Results The concentration of ESAT-6 in the CSF of TBM group was 4.46 pg/ml[(2.20-10.55) pg/ml]and the IFN-γwas 34.86 ng/L[(25.62-241.71) ng/L].The average concentrations of ESAT-6 and IFN-γin the intracranial infection of non-TBM group and the control group were(1.18±0.49) pg/ml,(12.00±3.37) ng/L and(1.05±0.47) pg/ml.(14.58±3.46) ng/L,respectively. The levels of ESAT-6 and IFN-γwere significantly higher in the TBM group than in the intracranial infection of non-TBM group(H=35.695.P=0.000) and the control group(H=31.560.P=0.000),but were not significantly different between the intracranial infection of non-TBM group and the control group(t= 1.226,P=0.226;t=0.060,P=0.952.respectively).The Spearman rank correlation analysis showed positive correlation between ESAT-6 and IFN-γdetected in the TBM group(r_s=1.000,P=0.000). Conclusion The levels of ESAT-6 and IFN-γin the TBM group were significantly different from the intracranial infection of non-TBM group and the control group.The 2 indicators present certain correlation and can be helpful in the clinical diagnosis of TBM.更多还原