【摘要】 以粪产碱杆菌来源的青霉素G酰化酶为催化剂,酶法拆分苯乙酰消旋底物转化生成(S)-2-氨基-1-丁醇。通过对催化过程中加酶量、底物浓度、反应温度、pH进行优化,确定最适酶催化条件是pH 9.0,40℃,底物浓度100 mmol/L,酶量2 U/mL,反应体积80 mL。在最适反应条件下,当消旋底物转化率达40%时终止反应可获得较高的产物光学纯度(ee>99%)。更多还原

【Abstract】 With the source of Alcaligenes faecalis penicillin G acylase as catalyst,optically pure(S)-2-amino-1-butanol is obtained via enantioselective hydrolysis of N-phenylacetyl racemic 2-amino-1-butanol.The optimal conditions of catalytic process are as follows:2 U/mL of enzyme,100 mmol/L of substrate concentration,40℃ of reaction temperature,9.0 of pH.Under the optimal conditions,the higher optical purity(ee>99%) can be obtained when the racemic substrate conversion rate of 40% is achieved.更多还原