【摘要】 目的:探讨淋巴管生成因子LYVE-1及Prox-1的定量表达与宫颈癌淋巴结转移的相关性。方法:收集宫颈鳞癌手术标本24例,运用半定量RT-PCR技术对淋巴管生成因子LYVE-1和Prox-1进行分析,分别比较这两种因子在切除标本的肿瘤组织、癌旁组织中表达的差异。结果:Prox-1在肿瘤及癌旁组织表达分别为1.52±0.26和0.46±0.19,LYVE-1在肿瘤及癌旁正常组织的表达分别为1.37±0.21和0.56±0.12。Prox-1及LYVE-1在肿瘤组织的表达均高于癌旁组织(P均<0.01),与肿瘤的发生有关。并且它们的表达与淋巴结转移有相关性(P<0.01)。结论:Prox-1及LYVE-1表达升高与宫颈癌发生及淋巴结转移有一定关系,检测该指标对了解宫颈癌的侵袭和转移具有一定价值。更多还原

【Abstract】 Objective:To investigate the relevance between the expression of lymphangiogenesis factor and cervical lymph node metastasis.Method:This study has collected 24 cases of surgical specimens of cervical squamous cell carcinoma with lymph node metastasis,using semi-quantitatie reverse transcription PCR technique to analyse the expression of lymphangiogenic factor LYVE-1 and Prox-1,and compared the differential expression of these two factors in tumor and adjacent tissue respectively.Results:The relative expression of Prox-1 in tumor and tissue adjacent to the tumor were 1.52±0.26 and 0.46±0.19(P<0.01),of LYVE-1 were 1.37±0.21 and 0.56±0.12(P<0.01).They had higher expression in cervical tissues than adjacent tissues,related with the development of cervical cancer and lymph node metastasis.Conclusion:Prox-1 and LYVE-1 were correlated with cervical cancer and lymph node metastasis,suggesting that detection of LYVE-1 and Prox-1 may be valuable in the evaluation of invasiveness and metastasis of cervical cancer.更多还原