【摘要】 目的:探讨穴位注射对脑缺血的改善作用及其机制。方法:48只Wistar大鼠随机分为假手术组、模型组、穴位注射组。采用线栓法制备大鼠大脑中动脉缺血再灌注模型。穴位注射组分别在造模后6、12、24 h在"百会""大椎"穴进行复方当归注射液穴位注射。运用免疫组化法观察再灌注24 h后各组大鼠脑组织缺血区海马回Bcl-2、Bax蛋白表达情况,用全自动血流变仪测定全血黏度、红细胞聚集指数、红细胞刚性指数和红细胞变形指数。结果:与假手术组比较,模型组大鼠海马回细胞Bcl-2和Bax表达增高(P<0.05),全血黏度、红细胞聚集指数和刚性指数增高(P<0.05),红细胞变形指数降低(P<0.05);与模型组比较,穴位注射组大鼠海马回细胞Bcl-2表达水平增高(P<0.05),Bax表达水平降低(P<0.05),Bcl-2/Bax的表达比例增高(P<0.05),全血黏度、红细胞聚集指数和刚性指数明显降低(P<0.05),红细胞变形指数增高(P<0.05)。结论:穴位注射有明显减少脑梗死边缘区域凋亡相关蛋白的作用,其机制与Bcl-2表达增高,Bax表达降低,升高Bcl-2/Bax值和改善血液流变性有关。更多还原

【Abstract】 Objective To observe the effect of acupoint-injection on the expression of Bcl-2 and Bax in the cerebral tissue around the ischemic area in cerebral ischemia-reperfusion(CI/R) rats so as to investigate its mechanism underlying improvement of cerebral infarction.Methods Forty-eight Wistar rats were randomized into sham operation(sham),model and acupoint-injection groups(n=16/group).CI/R model was duplicated by occlusion of the middle cerebral artery for 2h and reperfusion for 24h."Baihui"(GV20) and "Dazhui"(GV14) were used for injection of Fufang Danggui Zhusheye(Compound Angelica-root Injectio,1000g/mL),immediately 6h,12h and 24h after modeling.Bcl-2 and Bax immunoactivity in the cerebral tissue around the ischemic area was assayed by immunohistochemistry.Blood viscosity including high,medium and low shearing rates,erythrocyte aggregation index(AI),rigidity index(RI) and deformity index(DI) were assayed by using a full-automatic hemorheolometer.Results In comparison with the sham group,the numbers of both Bcl-2 and Bax immunoreaction(IR) positive cells in the ischemic cerebral tissue in the model group were increased significantly(P<0.05).Compared with the model group,the number of Bcl-2 IR-positive cells was increased further apparently in the acupoint-injection group(P<0.05),while that of Bax IR-positive cells in the acupoint-injection group lowered considerably(P<0.05).The ratio of Bcl-2/Bax was decreased significantly in the model group than that in the sham group(P<0.05),and increased evidently in the acupoint-injection group than in the mo-del group(P<0.05).Regarding the parameters of hemorheology,compared to the sham group,the high,medium and low shear rates,erythrocyte AI and RI were increased significantly in the model group(P<0.05),while DI was decreased significantly(P<0.05).Compared to the model group,the high,medium and low shear rates,and erythrocyte AI and RI in the acupoint-injection group were down-regulated significantly,and DI of the acupoint-injection group was up-regulated markedly(P<0.05).Conclusion Acupoint-injection of Compound Angelica-root Injectio can function in up-regulating CI/R induced increase of cerebral Bcl-2 expression and suppressing CI/R induced increase of cerebral Bax expression,blood viscosity,and erythrocyte AI and RI,and inhibiting CI/R induced decrease of erythrocyte DI in CI/R rats,which may contribute to its effects in improving cerebral ischemia by resisting neuronal apoptosis and facilitating cerebral blood circulation.更多还原