【摘要】 目的:以不同刺激参数的捻转手法针刺自发性高血压大鼠(SHR)"太冲"穴,比较其降压效应及对血浆内皮素-1(ET-1)的影响,探讨针刺"太冲"穴治疗原发性高血压的最佳降压手法参数,同时为针刺手法量学提供有益依据。方法:选用SHR28只,随机分为模型组、轻刺激组、中刺激组和重刺激组(每组均7只),并以同体重正常血压SD大鼠7只作为正常组。3个针刺组分别以不同参数针刺大鼠双侧"太冲"穴,每次针刺5 min,每天1次,在针刺结束后15 min采用BP-6无创血压测量系统对大鼠进行血压测定,针刺到第7天采用放射免疫法检测各组大鼠血浆ET-1含量。轻、中、重3种针刺参数捻转角度、频率以及是否穿透足底皮肤分别为:0°、0次/min、穿透足底皮肤,180°、60次/min、不穿透足底皮肤,360°、120次/min、穿透足底皮肤。结果:与轻刺激、重刺激组比较,针后第2天,中刺激组的收缩压显著降低(P<0.01);第6、7天,中刺激组收缩压显著低于模型组和轻、重刺激组(P<0.01)。组内比,中刺激组的收缩压于刺激后第2、5、6、7天明显降低(P<0.01)。模型组血浆ET-1水平显著高于正常组(P<0.01);中刺激组血浆ET-1水平显著低于模型组(P<0.01);而轻刺激和重刺激组的ET-1浓度与模型组比较,差异无统计学意义(P>0.05)。结论:"太冲"穴不同量化参数的针刺降压效应具有差异性,"太冲"穴中等强度刺激可降低高血压大鼠血压及血浆内皮素含量。更多还原

【Abstract】 Objective To observe the effect of different strength of acupuncture stimulation on blood pressure and plasma endothelin(E)-1 in spontaneous hypertension rats(SHR),so as to seek a better acupuncture parameter for clinical treatment of hypertension.Methods Twenty-eight 9-week-old SHRs were randomized into mild-stimulation group,moderate-stimulation group,strong-stimulation group and model group(n=7 in each group).Seven normotensive SD rats served as a normal control group.Acupuncture stimulation with mild,moderate and strong stimulation was applied to bilateral "Taichong"(LR3) for 5 min,once daily for 7 days.Blood pressure(BP) was determined by using a non-invasive BP-6 detection system.Plasma ET-1 was assayed by radioimmunoassay.Results Compared with the model group,the systolic pressure of the moderate-stimulation group on the 6th and 7th day was decreased significantly after acupuncture of "Taichong"(LR 3)(P<0.01),being significantly lower than that of the mild-and strong-stimulation groups(P<0.01).In comparison with the normal control group,plasma ET-1 level in the model group was increased significantly(P<0.01),while compared with the model group,only that in the moderate-stimulation group was down-regulated considerably(P<0.01).No significant differences were found between the mild-stimulation and model groups,between the strong-stimulation and model groups,and between the mild-stimulation and strong-stimulation groups in plasma ET-1 level(P>0.05).Conclusion Moderate-stimulation of "Taichong"(LR3) can lower blood pressure and plasma EA-1 level in spontaneous hypertension rats.The reduced level of plasma ET-1 may be one of its mechanisms underlying improving hypertension.更多还原