【摘要】 采用CCK-8细胞增殖检测、流式细胞仪、Hoechst 33258染色、细胞周期检测、细胞凋亡检测等研究了二氢青蒿素对人成骨肉瘤MG-63细胞凋亡的诱导作用。细胞增殖检测发现二氢青蒿素对MG-63细胞48h及72h时的50%抑制率分别为44.87μmol/L和19.15μmol/L;细胞周期检测发现细胞生长被阻滞在G₀/G₁期;Hoechst33258染色凋亡检测发现细胞核出现致密的固缩形态及颗粒状荧光;Annexin V-FITC双染细胞凋亡检测发现二氢青蒿素对MG-63细胞的凋亡率为45.10%。表明:二氢青蒿素可明显诱导MG-63细胞的凋亡,并且MG-63细胞的凋亡与二氢青蒿素存在剂量与时间依赖关系,二氢青蒿素对人成骨肉瘤MG-63细胞凋亡的诱导具有显著作用,这将为进一步研究细胞凋亡机制提供基础和实验依据。更多还原

【Abstract】 The apoptosis effects of dihydroartemisinin（DHA） on human osteosarcoma MG-63 cells have been studied using the methods of CCK-8 staining,flow cytometry analysis,Hoechst33258 staining,Annexin VFITC staining and electron microscope.The results showed that DHA decreased the cell viability in a concentration and time-dependent manner.The IC₅₀ values of DHA against the MG-63 cells were determined as 44.87μmol/L and 19.15μmol/L after 48 h and 72 h,respectively.The results of flow cytometry analysis showed that DHA could induce the emergence of the phase of apoptosis,the rate is 45.10%and the cell cycle is blocked in G₀/G₁ phase.Cell nucleus concentrated and appeared granular fluorescence by Hoechst33258 staining.This study suggested that DHA had induced apoptosis of the human osteosarcoma MG-63 cells effectively,and provided foundation and research proofs to study more about the apoptosis mechanisms of the osteosarcoma cells.更多还原