【摘要】 <正>黄瓜是我国栽培面积最大的喜温蔬菜之一。在生产上,高温干旱等环境胁、迫因子严重危害黄瓜的产量和品质,甚至导致绝收。任安芝等综述了SOD与水分、盐分、低温和大气污染等逆境胁迫之间的关系,认为:各种逆境胁迫引起的活性氧代谢平衡失调、生物膜结构破坏是导致植物遭受逆境伤害的机理之一。通过基因工程提高植物体内抗氧化酶活性及增加抗氧化代谢的水平是增强植物抗逆性的有效更多还原

【Abstract】 In this study,several factors affecting transformation rate were studied by Agrobacterium-mediated in Nong cheng No.3 cucumber.These factors included sensitivity of cotyledonary nodes to antibiotics （Kan and Cef）,days of pre-culture,concentrations of Agrobacterium tumefaciens,days of co-culture and time of being infected on the optimum culture medium and so on.The high frequency transgenic regeneration system of cucumber was established as follows:cotyledons nodes of Nong cheng No.3 cucumber were pre-cultured for 3 days, inoculated with EHA-105(D₆₀₀03,AS 10 mg/100 mL) for 5 minutes,then co-cultured for 2 days,transformed to screening-culture medium（50 mg/L Kan,500 mg/L Cef）,resistant buds grew well in light.9 resistant plants were obtained and the same positive bands of 658 bp were detected by examining GUS report gene and PCR amplification of Mn-SOD gene.So the integration of the gene into cucumber genome DNA was confirmed in some degree.更多还原