【摘要】 目的建立了一种简单、快速分析天山花楸果实中苦杏仁苷含量测定的胶束毛细管电泳法(MEKC)。方法采用熔融石英毛细管(58.5 cm×75μm,有效长度为48.5 cm),缓冲体系为70 mmol.L-1的硼砂-200 mmol.L-1的十二烷基硫酸钠(pH 9.50);分离电压10 kV(+)→(-);电泳时间40 min;电泳温度25℃;压力进样0.5 psi×10 s;柱上检测UV 215 nm(二极管阵列检测器)。结果苦杏仁苷在20 min内得到较好分离,在25~225μg.mL-1范围内与峰面积线性关系良好,线性方程为:Y=776.66X-6 637.9(r=0.999 7),样品平均回收率为100.8%,RSD值为2.8%。结论该方法简单、快速,结果准确可靠,可用于天山花楸中氰苷类成分测定及质量控制。更多还原

【Abstract】 Objective To establish a simple and rapid micellar electrokinetic chromatography method(MEKC) which can determine the content of amygdalin in Sorbus tianschanica Rupr.fruit.Methods Bare fused-silica capillary(58.5 cm× 75 μm,effective length of 48.5 cm) was used;the buffer was composed of 70 mmol·L-1 sodium borate-200 mmol·L-1 SDS(pH9.50);running voltage at 10 kV;polarity from anode(inlet) to cathode(outlet);separation time 40 min;temperature 25 ℃;sampling 0.5 psi×10 s;on line detection at 215 nm(PDA).Results The linear range of amygdalin was from 25-225 μg·mL-1,with linear regression equation was Y=776.66 X-6 637.9(r=0.999 7),and the average recovery was 100.8%,with RSD 2.8%.Conclusion The method is simple,rapid,accurate and reliable,and can be applied for the quality control of cyanogenic glycosides.更多还原