【摘要】 以气相色谱内标法测定变异黄芪中苦马豆素的含量。用超声波协助提取并用溶剂萃取变异黄芪样品,选用D-甘露醇为内标物,采用AT.SE-54毛细管作为色谱柱,柱温210℃,气化室300℃,检测器280℃,分流比30∶1,氢火焰离子化检测器。结果表明,苦马豆素与内标分离良好,在0.015 6~1.0 g/L时线性关系良好(R2=0.999 7),平均加样回收率为90.6%(RSD=2.41%)。该方法简便、灵敏、易操作,可用于疯草类植物样品中苦马豆素含量的检测。更多还原

【Abstract】 To develop a reliable method for determination of swainsonine of Astragalus variabilis Bunge by gas chromatography(GC),swainsonine was extracted with ultrasound-assisted liquid extraction method.AT.SE-54 capillary column was used and temperature was 210℃,injection temperature was 300℃,detector temperature was 280℃,splitting ratio was 30∶1,FID detector,the internal standard was D-Mannito.Swainsonine and D-Mannito were well separated,the standard curve was linear in the concentration range of 0.015 6-1.0 g/L(R2=0.999 7).The average recovery was 90.6%(RSD=2.41%).The method can be used to determine swainsonine of locoweed with high sensitivity,precise and good reproducibility.更多还原