【摘要】 目的:探讨姜黄素促人胃癌BGC-823细胞凋亡的生物学作用及其调控机制。方法:常规体外培养对数生长期胃癌BGC-823细胞,细胞分为对照组,低、中、高姜黄素处理组四组,姜黄素浓度分别为0 mg/L,5 mg/L,10 mg/L,20mg/L。姜黄素处理24 h后,采用甲基噻唑(MTT)比色法及流式细胞仪测定细胞增殖水平及细胞凋亡率;采用免疫组化法测定细胞内Bax、Bcl-2蛋白表达;采用PCR检测Caspase-3的mRNA表达水平。结果:MTT检测显示姜黄素能抑制人胃癌BGC-823细胞増殖,呈现浓度依赖性;流式细胞仪显示姜黄素能有效诱导细胞的凋亡,呈现浓度依赖性,其中20 mg/L姜黄素处理24 h后细胞凋亡率为48.3%;免疫组化试验表明姜黄素处理使人胃癌BGC-823细胞中Bax表达水平上调,同时Bcl-2蛋白表达水平下调;且细胞中Caspase-3的mRNA表达水平受姜黄素诱导而增高。结论:姜黄素对人胃癌BGC-823细胞的增殖具有明显抑制作用,呈浓度依赖性促进细胞凋亡,这种生物学效应可能与激活Bax蛋白表达、抑制Bcl-2蛋白表达而活化Caspase-3的信号通路有关。该研究为深入探讨姜黄素诱导人胃癌BGC-823细胞凋亡的机制提供了重要依据。更多还原

【Abstract】 AIM: discuss the biological function and regulation mechanism of curcumin in promoting human gastric carcinoma BGC-823 apoptosis.METHODS: Conventional in virto culture in logarithmic phase gastric carcinoma BGC-823 cells;cells are divided into four groups: control group,low treatment group,middle treatment group and high treatment group,with curcumin concentration being 0 mg/L,5 mg/L,10 mg/L,and 20 mg/L,respectively.24 hours after curcumin is treated,cell proliferation level and apoptosis rate are measured with MTT colorimetry and flow cytometry,Bax,Bcl-2 protein expression is measured with immunohistochemistry;mRNA of Caspase-3 is tested by means of PCR.RESULTS: MTT test indicates that curcumin can inhibit human gastric carcinoma BGC-823 cell proliferation,showing concentration dependency;flow cytometry shows that curcumin can effectively induce apoptosis,showing concentration dependency,where the apoptosis rate is 48.3% 24 hours after 20 mg/L curcumin is treated;immunohistochemistry test shows that curcumin treatment enables Bax expression level in human gastric carcinoma BGC-823 cells to go up,meanwhile,the Bcl-2 protein expression level to go down,besides,the mRNA expression level of Caspase-3 in cells increases through induction of curcumin.CONCLUSION: Curcumin has obvious inhibitory effect on human gastric carcinoma BGC-823 cell proliferation,showing concentration dependency to promote apoptosis.Such biological effect may be associated with activating Caspase-3 signal channel by activating Bax protein expression and inhibiting Bcl-2 protein.This study lays an important foundation for further discussing the mechanism of curcumin in inducing human gastric carcinoma BGC-823 apoptosis.更多还原