【摘要】 目的:观察过氧化物酶体增殖物激活型受体γ(PPARγ)的激动剂ciglitazone对THP-1巨噬细胞CD36表达及细胞胆固醇流入的影响。方法:以THP-1巨噬细胞为研究对象,用50 mg/L氧化低密度指蛋白(oxLDL)分别与不同浓度的PPARγ激动剂ciglitazone(0、5、10、15μmol/L)共同孵育THP-1巨噬细胞24 h后,采用液体闪烁计数法检测[3H]胆固醇流入,采用逆转录-聚合酶链式反应和Western blot分别检测CD36的表达。结果:oxLDL及不同浓度的ciglitazone与THP-1巨噬细胞共孵育后,与对照组比较(20.3%),加ciglitazone的处理组胆固醇流入随着浓度的增加而依次增加,分别为28.6%、37.2%、44.3%、48.7%,细胞CD36的表达上调。结论:ciglitazone可上调THP-1巨噬细胞CD36的表达,并增加细胞胆固醇流入。更多还原

【Abstract】 AIM: To investigate the effect of ciglitazone on CD36 expression and cholesterol influx in THP-1 macrophage.METHODS: After exposure of the cultured THP-1 macrophage to ciglitazone for 24 h,labeled Cholesterol influx was determined by FJ-2107P type liquid scintillator.CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting respectively.RESULTS: PPARγ agonist,ciglitazone,elevated CD36 in both protein and mRNA levels,and increased cholesterol influx in THP-1 macrophage.The levels of cholesterol influx were 20.3%,28.6%,37.2%,44.3%,48.7% respectively.CONCLUSION: Our results indicated that ciglitazone may play an important role in cholesterol influx and modulating CD36 expression in THP-1 macrophage.更多还原