【摘要】 从马铃薯中纯化一种天冬氨酸蛋白酶抑制剂(PPI)并对其与DNA的相互作用及抗氧化作用进行研究。以溴化乙啶作为探针,利用荧光法在初步筛选抗癌药物上的应用,首次对PPI与DNA的相互作用进行了研究;利用二苯代苦味酰基自由基体系、H2O2/Fe2+产生的羟基自由基体系、超氧阴离子自由基体系对PPI的体外清除自由基的能力进行研究,结果表明PPI具有一定体外抗氧化活性且从与DNA作用的角度分析可能具有抗癌效果。更多还原

【Abstract】 The purpose of this study was to purify a kind of aspartic protease inhibitor from potato and investigate its interaction with DNA and antioxidant activity.With ethidium bromide as a probe,Fluorescence method for screening anticancer drugs in the application,the first time the interaction between PPI and DNA has been studied;Use scavenging of free radicals of flavonoids,DPPH,H2O2/Fe2+ for hydroxyl radical system and superoxide anion radical system of the PPI in vitro antioxidant activity study,The results show that PPI has some antioxidant activity in vitro and from the point of view with the DNA may have anticancer effects.更多还原