【摘要】 目的建立一种测定保健食品中左旋肉碱的脉冲安培检测-高效阴离子色谱新方法。方法样品经乙醚溶解分散,用1.0mmol/L盐酸萃取其中的左旋肉碱后,上清液进离子色谱仪测定。以200mmmol/LNaOH为流动相,采用Amino PAC PA10氨基酸分离柱分离,Au为工作电极的脉冲安培检测器检测。结果左旋肉碱在0.08～1.00mg/ml浓度范围内线性关系良好,标准溶液测定的峰面积和保留时间的RSD为3.97%和0.62%,日间RSD为5.66%和0.69%。将此法用于保健食品样品分析,样品平均加标回收率为95.6%～113.5%,样品测定结果的RSD为7.34%。结论该方法简便准确,重现性好,15min可完成一次分析,适于保健品中左旋肉碱的定量快速检测。更多还原

【Abstract】 Objective A pulsed amperometric detector-high performance anion chromatography method for the determination of L-carnitine in health food was established.Methods After samples were dissolved and dispersed with ether,L-carnitine in samples was extracted with 0.1mmol/L HCl.Using 200mmol/L NaOH as the mobile phase,amino PAC PA10 Amino acid-separation column as separation column and pulse ampere detector with Au as working electrode throughout the experiment.Results The linear was good in the range of 0.08-1.00mg/ml.The intra-day RSDs of peak area and retention time for L-carnitine standard solution were 3.97% and 0.62% and the inter-day RSDs were 5.66% and 0.69%,respectively.The average recoveries were 95.6%-113.5% and the RSD of sample analysis was 7.34%.The results of the proposed method for the analysis of L-carintine in health foods were satisfactory.Conclusion The proposed method is simple,accurate and reproducible.One analysis can be completed within 15min.The method is adaptive for the analysis of L-carintine in health food.更多还原