【摘要】 目的探讨黄曲霉毒素G1(AFG1)对体外培养的人食管鳞状上皮细胞表面HLA-Ⅰ分子表达的影响。方法采用原代培养、免疫印迹(Western blot)和反转录聚合酶链反应(RT-PCR)分析方法研究不同浓度AFG1(50、100、1000和2000μg/L)处理后人食管鳞状上皮细胞表面HLA-Ⅰ分子表达的变化。结果免疫印迹结果表明,经不同浓度AFG1(50、100、1000、2000μg/L)处理细胞24h后,100、1000、2000μg/L AFG1处理组HLA-ABC的蛋白表达均明显低于溶剂对照组(P<0.05),而且在100～2000μg/L浓度范围内,人食管鳞状上皮细胞表面HLA-Ⅰ分子的表达逐渐降低,两者呈明显的负相关关系(r=-0.921,n=3,P<0.01)。RT-PCR检测在mRNA水平进一步证实了AFG1对HLA-ABC表达的影响。其中100、1000、2000μg/L AFG1处理细胞后,HLA-A mRNA的表达明显低于溶剂对照组(P<0.05);2000μg/L处理组HLA-B mRNA的表达较溶剂对照组降低(P<0.05);而各AFG1处理组HLA-C mRNA的表达没有明显影响。结论 AFG1处理可以抑制人食管鳞状上皮细胞表面HLA-Ⅰ分子的表达。更多还原

【Abstract】 Objective To explore the effect of AFG1 on the expression of human leukocyte antigen(HLA-I) molecule in human esophageal epithelial cells.Method Western Blot and RT-PCT analysis was used to explore the effect of AFG1 on the antigen presenting function of primary cultured esophageal epithelial cells.Results The protein expression of HLA-ABC was significantly decreased in the groups treated with 100,1000 and 2000μg /L AFG1 as compared with the solvent control group(P < 0.05).Within the range of 100-2000μg /L AFG1,the protein expression level of HLA-ABC of esophageal epithelial cells decreased gradually,and showed significant negative correlation(r =-0.921,n = 3,P < 0.01).The effect of AFG1 on the expression of HLA-ABC mRNA was further confirmed by RT-PCR analysis.The results showed that after treated with 100μg /L,1 000μg /L and 2 000 μg/L AFG1,the expression of HLA-A mRNA was decreased significantly as compared with the solvent control(P < 0.05),and the expression of HLA-B mRNA was decreased in the 2000μg /L AFG1 treated group(P < 0.05),while there was no effect on the expression of HLA-C mRNA.Conclusion The expression of HLA-Ⅰmolecule in human esophageal epithelial cells could be inhibited by AFG1 treatment.更多还原